Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture , Yancheng Teachers University , Yancheng 224051 , China.
Key Laboratory of Biotechnology in Lianyungang Normal College , Lianyungang 222006 , China.
J Agric Food Chem. 2018 Mar 21;66(11):2598-2606. doi: 10.1021/acs.jafc.7b05074. Epub 2018 Mar 6.
Cr (VI), the pathogenicity factor, is widely known to cause toxic effects in living organisms. Given the economic importance of the mantis shrimp ( Oratosquilla oratoria), the understanding of impacts by Cr (VI) is considered important. In this study, transcriptome of mantis shrimp was characterized by a comparison between control and Cr (VI)-treated samples using RNA-seq approach. Totally, 88 234 826 bp and 13.24G clean reads were obtained. The total length and number of unigenes were 68 411 206 bp and 100 918, respectively. The maximal and average length of unigenes was 24 906 bp and 678 bp, respectively (N50, 798 bp). 7115 of these unigenes accounted for 7.05% of the total that were annotated in all databases. After annotation of assembled unigenes, 35 619 of them were assigned into 3 functional categories and 56 subcategories using Gene Ontology; 18 580 of them were assigned into 26 functional categories using Clusters of Orthologous Groups of proteins; 16 864 of them were assigned into 5 major categories and 32 subclasses using KEGG. Finally, 1730 genes were differentially expressed (DGEs), 9 up-regulated pathways (protein digestion and absorption, neuroactive ligand-receptor interaction, pancreatic secretion, tyrosine metabolism, amoebiasis, ECM-receptor interaction, riboflavin metabolism, amino sugar and nucleotide sugar metabolism and AGE-RAGE signaling pathway in diabetic complications) were significantly enriched ( q < 0.05), and one down-regulated pathway ( Staphylococcus aureus infection) was significantly enriched ( q < 0.05). Up-regulation of genes in pathways of protein digestion/absorption ( PepT1/SLC15A and ATP1B) and environment information processing ( COL1AS, COL4A; LAMA3_5, LAMB3; FN1 and TN) may imply the potentially positive toxicity resistance mechanisms.
Cr（VI）是一种致病因子，被广泛认为会对生物体造成毒性影响。鉴于螳螂虾（Oratosquilla oratoria）的经济重要性，了解 Cr（VI）的影响被认为很重要。在这项研究中，通过 RNA-seq 方法比较对照和 Cr（VI）处理样品，对螳螂虾的转录组进行了表征。总共获得了 88,234,826bp 和 13.24G 的清洁读数。总长度和基因数量分别为 68,411,206bp 和 100,918，最长和平均基因长度分别为 24,906bp 和 678bp（N50，798bp）。这些基因中有 7,115 个占总基因的 7.05%，在所有数据库中都有注释。在对组装的基因进行注释后，根据基因本体论（GO），将 35,619 个基因分为 3 个功能类别和 56 个亚类；根据蛋白质同源物聚类（COG），将 18,580 个基因分为 26 个功能类别；根据京都基因与基因组百科全书（KEGG），将 16,864 个基因分为 5 个主要类别和 32 个亚类。最后，有 1730 个基因差异表达（DGE），9 个上调途径（蛋白质消化吸收、神经活性配体-受体相互作用、胰腺分泌、酪氨酸代谢、变形虫病、细胞外基质-受体相互作用、核黄素代谢、氨基糖和核苷酸糖代谢和糖尿病并发症中的 AGE-RAGE 信号通路）显著富集（q<0.05），一个下调途径（金黄色葡萄球菌感染）显著富集（q<0.05）。蛋白质消化/吸收途径（PepT1/SLC15A 和 ATP1B）和环境信息处理途径（COL1AS、COL4A；LAMA3_5、LAMB3；FN1 和 TN）中基因的上调可能暗示了潜在的积极毒性抵抗机制。
