Lou Fangrui, Gao Tianxiang, Cai Shanshan, Han Zhiqiang
Fisheries College, Ocean University of China, Qingdao 266003, China.
Fishery College, Zhejiang Ocean University, Zhoushan 316022, China.
Mar Genomics. 2018 Apr;38:17-20. doi: 10.1016/j.margen.2017.08.003. Epub 2017 Sep 1.
As the representative species of Stomatopoda, Oratosquilla oratoria can tolerate the complex benthic environment. In this study, our goal was to develop the transcriptomic resource of O. oratoria that would support adaptation mechanism studies. We generated the whole transcriptome of O. oratoria from combined tissues (eyestalk, muscle, sexual and viscus) using Hiseq technology. A total of 51,305,284 high-quality clean reads were assembled to produce 59,054 non-redundant transcripts with a mean length of 987nt using Trinity and CORSET software. Among the predictable unigenes, a total of 32,451 unigenes were annotated based on protein databases. Finally, we predicted the coding sequences of 31,822 unigenes and obtained 19,057 SSRs in the present study. The present study will provide an important resource and foundational understanding for future genomic research of O. oratoria.
口虾蛄作为口足目代表性物种，能够耐受复杂的底栖环境。在本研究中，我们的目标是开发口虾蛄的转录组资源，以支持对其适应机制的研究。我们使用Hiseq技术从组合组织（眼柄、肌肉、性腺和内脏）中生成了口虾蛄的全转录组。使用Trinity和CORSET软件对总共51,305,284条高质量的 clean reads进行组装，得到了59,054条非冗余转录本，平均长度为987nt。在可预测的单基因中，基于蛋白质数据库总共注释了32,451个单基因。最后，我们预测了31,822个单基因的编码序列，并在本研究中获得了19,057个SSR。本研究将为口虾蛄未来的基因组研究提供重要资源和基础认识。
