Arokiaraj M C, Menesson E, Feltin N
Cardiology, Pondicherry Institute of Medical Sciences, 605001 Pondicherry, India.
Tebu-Bio France, 39, rue de Houdan, 78610 Le Perray-en-Yvelines, France.
J Med Vasc. 2018 Feb;43(1):10-19. doi: 10.1016/j.jdmv.2017.11.002. Epub 2017 Dec 18.
Contrast-induced nephropathy is a commonly encountered problem in clinical practice. The purpose of the study was to design and develop a novel contrast agent, which could be used to prevent contrast-induced nephropathy in the future.
In total, 20-220nm magnetic nanoparticles were conjugated with iodixanol, and their radio-opacity and magnetic properties were assessed thereafter. Scanning electron microscopy pictures were acquired. Thereafter, the nanoparticles conjugate was tested in cell culture (HUVEC cells), and Quantibody assay was studied after cell treatment in 1:5 dilutions for 48h, compared with control.
The conjugate preparation had an adequate radio-opacity. A 4mm magnetic bubble was attached to a bar magnet and the properties were studied. The magnetic bubble maintained its structural integrity in all angles including antigravity position. Scanning electron microscopy showed magnetic nanoparticles in all pictures and the particles are of 100-400nm agglomerates with primary particle sizes of roughly 20nm. 1:5 diluted particles had no effect on secretion of IL-1a, IL-1b, IL-4, IL-10, IL-13 and TNFa. Particles increased secretion of IL-8 from 24h and 48h. Secretion of IFNg was also increased when particles were added to the cells as early as 1h. Likewise, IL-6 was strongly secreted by HUVEC treated with particles from 24h incubation time. In contrast, the secretion of MCP-1 was slightly reduced on HUVEC treated with particles.
There is potential for a novel iodixanol-magnetic nanoparticle conjugate to be used in cineradiography. Further investigations need to be performed to study its performance in vitro and in vivo.
对比剂肾病是临床实践中常见的问题。本研究的目的是设计并开发一种新型造影剂，以便未来用于预防对比剂肾病。
将20 - 220nm的磁性纳米颗粒与碘克沙醇偶联，随后评估其造影性和磁性。获取扫描电子显微镜图像。之后，在细胞培养（人脐静脉内皮细胞）中测试纳米颗粒偶联物，并在细胞以1:5稀释处理48小时后进行定量抗体检测，与对照组进行比较。
偶联物制剂具有足够的造影性。一个4mm的磁泡附着在条形磁体上并研究其性质。磁泡在所有角度包括反重力位置都保持其结构完整性。扫描电子显微镜显示所有图像中均有磁性纳米颗粒，颗粒为100 - 400nm的团聚体，初级粒径约为20nm。1:5稀释的颗粒对IL - 1α、IL - 1β、IL - 4、IL - 10、IL - 13和TNFα的分泌没有影响。颗粒从24小时和48小时起增加IL - 8的分泌。当颗粒早在1小时就添加到细胞中时，IFNγ的分泌也增加。同样，从24小时孵育时间起，用颗粒处理的人脐静脉内皮细胞强烈分泌IL - 6。相比之下，用颗粒处理的人脐静脉内皮细胞上MCP - 1的分泌略有减少。
新型碘克沙醇 - 磁性纳米颗粒偶联物有用于荧光电影摄影术的潜力。需要进一步研究以考察其在体外和体内
