Tekletsion Yenenesh K, Christensen Hannah, Finn Adam
School of Cellular and Molecular Medicine, University of Bristol, BS8 1TD Bristol, UK.
Population Health Sciences, Bristol Medical School, University of Bristol, BS8 2BN Bristol, UK.
J Microbiol Methods. 2018 Mar;146:100-103. doi: 10.1016/j.mimet.2018.02.003. Epub 2018 Feb 7.
Detection of bacterial gene transcripts in low density mucosal samples is challenging. We evaluated the NanoString nCounter system for transcript detection in Neisseria meningitidis (Nm) cultures. The method was sensitive, reproducible (R = 0.99) and demonstrated changes in gene expression. Studying Nm transcripts from pharyngeal samples may be feasible using this approach.
在低密度黏膜样本中检测细菌基因转录本具有挑战性。我们评估了NanoString nCounter系统用于检测脑膜炎奈瑟菌（Nm）培养物中的转录本。该方法灵敏、可重复（R = 0.99），并显示出基因表达的变化。使用这种方法研究来自咽部样本的Nm转录本可能是可行的。
