MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, UK.
LABGeM, Génomique Métabolique, CEA, Genoscope, Institut François Jacob, Université d'Evry, Université Paris-Saclay, CNRS, Evry, France.
Nat Commun. 2020 Nov 2;11(1):5541. doi: 10.1038/s41467-020-19347-y.
The bacterium Neisseria meningitidis causes life-threatening meningitis and sepsis. Here, we construct a complete collection of defined mutants in protein-coding genes of this organism, identifying all genes that are essential under laboratory conditions. The collection, named NeMeSys 2.0, consists of individual mutants in 1584 non-essential genes. We identify 391 essential genes, which are associated with basic functions such as expression and preservation of genome information, cell membrane structure and function, and metabolism. We use this collection to shed light on the functions of diverse genes, including a gene encoding a member of a previously unrecognised class of histidinol-phosphatases; a set of 20 genes required for type IV pili function; and several conditionally essential genes encoding antitoxins and/or immunity proteins. We expect that NeMeSys 2.0 will facilitate the phenotypic profiling of a major human bacterial pathogen.
脑膜炎奈瑟菌会引起危及生命的脑膜炎和败血症。在这里，我们构建了该生物体中蛋白质编码基因的完整定义突变体集合，确定了所有在实验室条件下必不可少的基因。该集合命名为 NeMeSys 2.0，由 1584 个非必需基因的单个突变体组成。我们鉴定出 391 个必需基因，这些基因与基本功能相关，例如基因组信息的表达和保存、细胞膜结构和功能以及新陈代谢。我们使用该集合来阐明各种基因的功能，包括一个编码先前未被识别的组氨酸醇磷酸酶成员的基因；一组 20 个基因，这些基因对于 IV 型菌毛功能是必需的；以及几个条件必需基因，这些基因编码抗毒素和/或免疫蛋白。我们预计 NeMeSys 2.0 将有助于对主要人类细菌病原体进行表型分析。
