Gough I R, Finnimore M
Aust N Z J Surg. 1979 Dec;49(6):716-20. doi: 10.1111/j.1445-2197.1979.tb06501.x.
The hypothesis that tissue culture alters the immunogenicity of grafts by removal or inactivation of passenger leucocytes has been investigated in an inbred rat parathyroid allograft model. DA rat (Ag-B4) parathyroid glands cultured for up to three weeks in Eagle's minimal essential medium (MEM) alone, or MEM with added donor-specific antilymphocyte serum, did not survive longer than fresh glands when allografted into Lewis rat (Ag-B1) recipients. Subsequent in vitro comparisons of three different culture media established that RPMI 1640 was superior to MEM and Medium 199 for rat parathyroids. However, further series of transplants after culture in RPMI 1640 alone also failed to produce prolongation of allograft survival.
通过去除或灭活过客白细胞，组织培养会改变移植物免疫原性的这一假说，已在近交系大鼠甲状旁腺同种异体移植模型中进行了研究。将DA大鼠（Ag-B4）的甲状旁腺在伊格尔氏最低限度基本培养基（MEM）中单独培养长达三周，或在添加了供体特异性抗淋巴细胞血清的MEM中培养，当将其同种异体移植到刘易斯大鼠（Ag-B1）受体中时，其存活时间并不比新鲜腺体更长。随后对三种不同培养基进行的体外比较表明，RPMI 1640培养基对大鼠甲状旁腺而言优于MEM和199培养基。然而，仅在RPMI 1640中培养后进行的进一步系列移植也未能延长同种异体移植物的存活时间。
