Starling J R, Fidler R, Corry R J
Surgery. 1977 Jun;81(6):668-75.
To determine whether survival of allografted rat parathyroid tissue could be enhanced either by the administration of antidonor serum (enhancing serum) or by tissue culture of the graft, parathyroids from Lewis x Brown Norway F1 (LBNF1) rats were transplanted beneath the fascia and between hamstring muscle fibers of hypocalcemic parathyroidectomized Lewis recipients (serum calcium 4.3 +/- 0.13mg%--mean +/- SEM). A drop in serum calcium below 7.5 mg% indicated rejection. Seven group 1 rats that received isogeneic parathyroids recovered from their hypocalcemic state and remained normocalcemic indefinitely. Seven group 2 rats that received LBNF(1) parathyroids experienced a reduced mean maximal response (MMR) of calcium increase of 4.1 +/- 0.2 mg% and the grafts had a median survival time (MST) of 16.0 +/- 1.32 days. Group 3 rats that received 1 ml of enhancing serum by intraperiotoneal injection, cytotoxic titer 1:128, on alternate days for five doses experienced an MMR of 5.0 +/- 0.7 mg% and had an MST of 19.3 +/- 1.07 days. When enhancing serum, cytotoxic titer 1:256, was administered to group 4, the MST of the grafts was increased to 29.7 +/- 1.19 days. When LBNF(1) parathyroid tissue was preserved in tissue culture for 14 to 21 days and then transplanted into Lewis recipients, the MST was 22.5 +/- 1.48 days. The administration of enhancing serum to recipients of cultured parathyroids further extended graft survival, MST 57.5 +/- 2.32 days. In summary, survival of allografted parathyroid tissue can be prolonged by the administration of enchancing serum or tissue culture of the graft. When enhancing serum was administered to recipients of cultured allografted parathyroid tissue, survival was further increased.
为了确定给予抗供体血清（增强血清）或对移植物进行组织培养是否能够提高同种异体移植大鼠甲状旁腺组织的存活率，将来自Lewis×Brown Norway F1（LBNF1）大鼠的甲状旁腺移植到低血钙的甲状旁腺切除Lewis受体（血清钙4.3±0.13mg%——平均值±标准误）的筋膜下和腘绳肌纤维之间。血清钙降至7.5mg%以下表明发生排斥反应。7只接受同基因甲状旁腺移植的第1组大鼠从低血钙状态恢复，并一直保持血钙正常。7只接受LBNF(1)甲状旁腺移植的第2组大鼠，钙增加的平均最大反应（MMR）降低了4.1±0.2mg%，移植物的中位存活时间（MST）为16.0±1.32天。第3组大鼠通过腹腔注射给予1ml增强血清（细胞毒性效价1:128），隔日注射5剂，其MMR为5.0±0.7mg%，MST为19.3±1.07天。当给第4组大鼠注射细胞毒性效价为1:256的增强血清时，移植物的MST增加到29.7±1.19天。当LBNF(1)甲状旁腺组织在组织培养中保存14至21天，然后移植到Lewis受体中时，MST为22.5±1.48天。给培养的甲状旁腺移植受体注射增强血清进一步延长了移植物存活时间，MST为5７.5±2.32天。总之，给予增强血清或对移植物进行组织培养可延长同种异体移植甲状旁腺组织的存活时间。当给培养的同种异体移植甲状旁腺组织的受体注射增强血清时，存活时间进一步延长。
