Chen Chun-Yen, Chang Yu-Han
University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
J Biosci Bioeng. 2018 Jun;125(6):710-716. doi: 10.1016/j.jbiosc.2018.01.008. Epub 2018 Feb 13.
Microalgae cultivation using wastewater is an approach for simultaneous wastewater treatment and biofuels/chemicals productions. In this study, three microalgae species Chorella vulgaris ESP-31, Chorella sorokiniana CY-1 and Scenedesmus sp. were cultivated using coke-making wastewater generated from a steel-making company. Of these, C.vulgaris ESP-31 had the best tolerance towards wastewater, with maximal biomass concentration of 2.82 g/l and lipid productivity of 32.3 mg/l/d. The highest biomass concentration 3.98 g/l and lipid productivity of 47.1 mg/l/d was obtained in 20% wastewater. Immobilization approach was applied to boost biomass growth and lipid production. Both maximal biomass growth (5.17 g/l) and lipid productivity (68.4 mg/l/d) was significantly enhanced with activated charcoal addition. Semi-batch cultivation resulted in stable biomass production and lipid productivity of 5.18 g/l and 77.3 mg/l/d, respectively. This study has revealed that C. vulgaris ESP-31 is a potential candidate for growth in coke making wastewater and biofuel production.
利用废水培养微藻是一种同时进行废水处理和生物燃料/化学品生产的方法。在本研究中，使用一家钢铁公司产生的焦化废水培养了三种微藻，即普通小球藻ESP-31、索氏小球藻CY-1和栅藻属。其中，普通小球藻ESP-31对废水的耐受性最佳，最大生物量浓度为2.82 g/l，脂质生产率为32.3 mg/l/d。在20%的废水中获得了最高生物量浓度3.98 g/l和脂质生产率47.1 mg/l/d。采用固定化方法促进生物量生长和脂质生产。添加活性炭后，最大生物量生长（5.17 g/l）和脂质生产率（68.4 mg/l/d）均显著提高。半连续培养实现了稳定的生物量生产，脂质生产率分别为5.18 g/l和77.3 mg/l/d。本研究表明，普通小球藻ESP-31是在焦化废水中生长和生物燃料生产的潜在候选微藻。
