Department of Mechanical Engineering, Faculty of Science and Technology, Keio University, Kohoku-ku, Yokohama, Japan.
Institute of Industrial Science, The University of Tokyo, Meguro-ku, Tokyo, Japan.
Biotechnol Bioeng. 2018 Jun;115(6):1614-1623. doi: 10.1002/bit.26559. Epub 2018 Mar 6.
This paper describes three-dimensional (3D) tissue shape control of mouse neural stem cell (mNSC) micro tissues by using closed agarose microchambers for effective differentiation induction of neurons in vitro. Our agarose microchambers, made by micromolding, can be sealed with an agarose sheet to form the mNSC tissues along the shape of microchambers. We constructed lane-shaped mNSC tissues with different width (∼60-210 μm) and thickness (∼25-95 μm) dimensions and induced differentiation to neurons with differentiation medium. We found that in thick tissues (thickness: >60 μm), distribution of differentiated neurons was not uniform, whereas in thin tissues (thickness: ∼30 μm), differentiated neurons were uniformly distributed with high differentiation efficiency. Our system to construct in vitro 3D neural tissues having uniformly distributed neurons at high differentiation ratio, could become an effective tool for drug screening using 3D neural tissues and 3D mNSC tissues under differentiation induction.
本文描述了通过使用封闭的琼脂糖微室来控制小鼠神经干细胞（mNSC）微组织的三维（3D）组织形状，从而在体外有效诱导神经元分化。我们的琼脂糖微室通过微成型制成，可以用琼脂糖片密封，形成沿微室形状排列的 mNSC 组织。我们构建了具有不同宽度（约 60-210μm）和厚度（约 25-95μm）尺寸的车道形 mNSC 组织，并使用分化培养基诱导其向神经元分化。我们发现，在较厚的组织（厚度：>60μm）中，分化神经元的分布不均匀，而在较薄的组织（厚度：约 30μm）中，分化神经元均匀分布，具有较高的分化效率。我们构建具有高分化率且神经元均匀分布的体外 3D 神经组织的系统，可能成为使用 3D 神经组织和分化诱导下的 3D mNSC 组织进行药物筛选的有效工具。
