Post-Graduation in Biotechnology, Renorbio/ Federal Rural University of Pernambuco, Rua Manoel de Medeiros, s/n - Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil.
Biotechnology Laboratory, Embrapa Algodão, Rua Osvaldo Cruz, 1143 - Centenário, 58428-095, Campina Grande, Paraíba, Brazil.
J Biotechnol. 2018 Mar 20;270:44-50. doi: 10.1016/j.jbiotec.2018.02.002. Epub 2018 Feb 7.
Substantial progress is being reported in the techniques for plant transformation, but successful regeneration of some genotypes remains a challenging step in the attempts to transform some recalcitrant species. GhSERK1 gene is involved on embryo formation, and its overexpression enhances the embryogenic competence. In this study we validate a short GhSERK1 probe in order to identify embryogenic cotton genotypes using RT-qPCR and blotting assays. Cotton genotypes with contrasting somatic embryogenic capacity were tested using in vitro procedures. High expression of transcripts was found in embryogenic genotypes, and the results were confirmed by the RT-PCR-blotting using a non-radioactive probe. The regeneration ability was confirmed in embryogenic genotypes. We confirmed that GhSERK1 can be used as marker for estimating the somatic embryogenesis ability of cotton plants.
在植物转化技术方面取得了重大进展，但在尝试转化一些顽固物种时，一些基因型的成功再生仍然是一个具有挑战性的步骤。GhSERK1 基因参与胚胎形成，其过表达可增强胚胎发生能力。在这项研究中，我们使用 RT-qPCR 和印迹分析验证了一个短的 GhSERK1 探针，以鉴定使用体外程序测试具有不同体细胞胚胎发生能力的棉花基因型。在胚胎发生基因型中发现了高表达的转录物，并且使用非放射性探针的 RT-PCR-印迹证实了结果。在胚胎发生基因型中证实了再生能力。我们证实 GhSERK1 可以用作标记物来估计棉花植物的体细胞胚胎发生能力。
