Pathobiological and Medical Diagnostics Department, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.
Department of Community Medicine, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.
J Microbiol Immunol Infect. 2019 Aug;52(4):563-570. doi: 10.1016/j.jmii.2018.01.003. Epub 2018 Jan 31.
Cholera is an important health problem in Sabah, a Malaysian state in northern Borneo; however, Vibrio cholerae in Sabah have never been characterized. Since 2002, serogroup O1 strains having the traits of both classical and El Tor biotype, designated as atypical El Tor biotype, have been increasingly reported as the cause of cholera worldwide. These variants are believed to produce clinically more severe disease like classical strains.
The purpose of this study is to investigate the genetic diversity of V.cholerae in Sabah and whether V.cholerae in Sabah belong to atypical El Tor biotype.
ERIC-PCR, a DNA fingerprinting method for bacterial pathogens based on the enterobacterial repetitive intergenic consensus sequence, was used to study the genetic diversity of 65 clinical V.cholerae O1 isolates from 3 districts (Kudat, Beluran, Sandakan) in Sabah and one environmental isolate from coastal sea water in Kudat district. In addition, we studied the biotype-specific genetic traits in these isolates to establish their biotype.
Different fingerprint patterns were seen in isolates from these three districts but one of the patterns was seen in more than one district. Clinical isolates and environmental isolate have different patterns. In addition, Sabah isolates harbor genetic traits specific to both classical biotype (ctxB-1, rstR) and El Tor biotype (rstR, rstC, tcpA, rtxC, VC2346).
This study revealed that V.cholerae in Sabah were genetically diverse and were atypical El Tor strains. Fingerprint patterns of these isolates will be useful in tracing the origin of this pathogen in the future.
沙巴是马来西亚在婆罗洲北部的一个州，霍乱是该地区的一个重要健康问题；然而，沙巴从未对霍乱弧菌进行过特征描述。自 2002 年以来，具有经典和埃尔托生物型特征的 O1 血清群菌株（被称为非典型埃尔托生物型）被越来越多地报道为全球霍乱的病因。这些变体被认为会导致比经典菌株更严重的临床疾病。
本研究旨在调查沙巴霍乱弧菌的遗传多样性，以及沙巴的霍乱弧菌是否属于非典型埃尔托生物型。
ERIC-PCR 是一种基于肠细菌重复基因间共有序列的细菌病原体 DNA 指纹图谱方法，用于研究来自沙巴三个地区（古达、伯鲁兰、山打根）的 65 株临床霍乱弧菌 O1 分离株和古达区沿海海水的一株环境分离株的遗传多样性。此外，我们研究了这些分离株中的生物型特异性遗传特征，以确定它们的生物型。
这三个地区的分离株显示出不同的指纹图谱模式，但有一种模式在不止一个地区出现。临床分离株和环境分离株具有不同的模式。此外，沙巴分离株携带经典生物型（ctxB-1、rstR）和埃尔托生物型（rstR、rstC、tcpA、rtxC、VC2346）特异性的遗传特征。
本研究表明，沙巴的霍乱弧菌具有遗传多样性，是非典型埃尔托菌株。这些分离株的指纹图谱模式将有助于追踪未来该病原体的来源。
