Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China.
Bioresour Technol. 2018 May;256:61-68. doi: 10.1016/j.biortech.2018.01.143. Epub 2018 Feb 2.
Potential of hydrolyzed polyacrylamide (HPAM) biodegradation to final products was studied through regulating its own nitrogen transformation. Under the conditions of 2, 3 and 4 mg/L of DO, HPAM removal ratio reached 16.92%, 24.51% and 30.78% and the corresponding removal ratio reached 49.15%, 60.25% and 76.44% after anaerobic biodegradation. NO-N concentration was 9.43, 14.10 and 17.99 mg/L in aerobic stages and the corresponding concentration was 0.17, 0.07 and 0.008 mg/L after anaerobic biodegradation. Oxygen as electron acceptors stimulated the activities of nitrification bacteria and other functional bacteria, thus further enhanced nitrification and HPAM biodegradation. NO (from HPAM oxidation) as electron acceptors stimulated the activities of nitrate-reducing, acetate-producing and methanogenic microorganisms and they could form a synergistic effect on denitrification and methanogenesis. Thermodynamic opportunity window revealed that NO could accelerate anaerobic HPAM bioconversion to methane. Aerobic and anaerobic growth-process equations of cells verified that the metabolism on HPAM was feasible.
通过调控自身氮转化，研究了水解聚丙烯酰胺（HPAM）向最终产物降解的潜力。在 2、3 和 4mg/L DO 条件下，厌氧生物降解后 HPAM 的去除率分别达到 16.92%、24.51%和 30.78%，相应的去除率分别达到 49.15%、60.25%和 76.44%。好氧阶段的 NO-N 浓度分别为 9.43、14.10 和 17.99mg/L，厌氧生物降解后相应的浓度分别为 0.17、0.07 和 0.008mg/L。氧气作为电子受体刺激了硝化细菌和其他功能细菌的活性，从而进一步增强了硝化和 HPAM 的生物降解。作为电子受体的 NO（来自 HPAM 的氧化）刺激了硝酸盐还原、乙酸产生和产甲烷微生物的活性，它们可以对反硝化和产甲烷产生协同作用。热力学机会窗口表明，NO 可以加速厌氧 HPAM 向甲烷的生物转化。细胞的好氧和厌氧生长过程方程验证了 HPAM 的代谢是可行的。
