Wang Yang, Yu Juan, Liu Lvjun, Li Wen, Duan Xingxiang, Peng Yingying, Zeng Sicong, Ouyang Qi, Lu Guangxiu, Lin Ge, Sun Yi
Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
National Engineering and Research Center of Human Stem Cells, Changsha, China.
Stem Cell Res. 2018 Apr;28:61-65. doi: 10.1016/j.scr.2018.01.020. Epub 2018 Feb 3.
The human embryonic stem cell (hESC) line NERCe003-A-1 was generated by introducing lentiviral-vector-mediated tetracycline-inducible β-catenin expression into a normal hESC line, NERCe003-A. The resulting cell line can overexpress the β-catenin protein, encoded by the CTNNB1 gene, after exposure to doxycycline (Dox). CTNNB1 gene expression was confirmed by quantitative PCR (qPCR) and immunofluorescence assays. Further characterization confirmed that the NERCe003-A-1 cell line expresses typical pluripotency markers and has the ability to form the three germ layers both in vitro and in vivo.
