Generation of a luciferase-expressing human embryonic stem cell line: NERCe002-A-2.
作者信息
Peng Yingying, Xie Menghan, Duan Xingxiang, Hu Liang, Yu Juan, Zeng Sicong, Wang Yang, Ouyang Qi, Lu Guangxiu, Lin Ge, Sun Yi
机构信息
Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China.
出版信息
Stem Cell Res. 2018 Apr;28:172-176. doi: 10.1016/j.scr.2018.02.010. Epub 2018 Feb 15.
The human embryonic stem cell line NERCe002-A-2 was generated by transduction of NERCe002-A cells with an expression vector carrying the luciferase gene. The stem cells labelled with luciferase can be transplanted into animals and detected by the bioluminescence imaging technology. This provides optimal prospects of application to in vivo stem cell tracing. Luciferin served as a substrate to detect the activity of luciferase, and luciferase expression was measured by quantitative PCR. Characterization assays suggested that the NERCe002-A-2 cell line expresses typical markers of pluripotency and can form the 3 germ layers in vivo.
Zotero 插件