Sule Nitesh, Penarete-Acosta Daniel, Englert Derek L, Jayaraman Arul
Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX, USA.
Department of Chemical and Materials Engineering, University of Kentucky, Paducah, KY, USA.
Methods Mol Biol. 2018;1729:47-59. doi: 10.1007/978-1-4939-7577-8_5.
Microfluidic technology allows fast and precise measurement of chemotaxis responses to both attractant and repellent signals. One of the major drawbacks of current microfluidic chemotaxis assays is the presence of bacterial cells within the concentration gradient flow field, which has the potential for flow effects masking the chemotaxis response. This chapter describes a new microfluidic device for producing stable concentration gradients and measuring the response of cells to the gradient without exposing them to any flow. Unlike other methods described in the literature, this method is capable of producing gradients of any shape, almost instantaneously, allowing the measurement of time-dependent response of cells to a variety of signals.
微流控技术能够快速、精确地测量细胞对趋化因子和驱化因子信号的趋化反应。当前微流控趋化分析的主要缺点之一是在浓度梯度流场中存在细菌细胞，这有可能导致流动效应掩盖趋化反应。本章介绍了一种新型微流控装置，该装置可产生稳定的浓度梯度，并在不使细胞暴露于任何流动的情况下测量细胞对梯度的反应。与文献中描述的其他方法不同，该方法能够几乎瞬间产生任何形状的梯度，从而能够测量细胞对各种信号的时间依赖性反应。
