Frank Vered, Vaknin Ady
The Racah Institute of Physics, The Hebrew University, Jerusalem, Israel.
Methods Mol Biol. 2018;1729:247-252. doi: 10.1007/978-1-4939-7577-8_20.
The anisotropy of the fluorescence emitted from fluorescent proteins, such as yellow fluorescent protein (YFP), is sensitive to Homo-FRET between the proteins. This effect can be used to detect in vivo ligand-induced changes in packing or conformation of tagged chemoreceptors. Such measurements of clustered or dispersed core-signaling units revealed quantitative dose-dependent responses of these sensors.
诸如黄色荧光蛋白（YFP）等荧光蛋白发出的荧光的各向异性对蛋白质之间的同源荧光共振能量转移（Homo-FRET）敏感。这种效应可用于检测体内配体诱导的标记化学感受器的堆积或构象变化。对聚集或分散的核心信号单元的此类测量揭示了这些传感器的定量剂量依赖性反应。
