Parales Rebecca E, Ditty Jayna L
Department of Microbiology and Molecular Genetics, College of Biological Sciences, University of California, Davis, CA, USA.
Department of Biology, College of Arts and Sciences, University of St. Thomas, St. Paul, MN, USA.
Methods Mol Biol. 2018;1729:255-280. doi: 10.1007/978-1-4939-7577-8_21.
Although the mechanism of bacterial chemotaxis has been extensively studied in enteric bacteria, the hunt for novel and atypical chemoeffectors (in enterics and distantly-related species alike) has necessitated the modification of classic chemotaxis assays to deal with recalcitrant and potentially toxic chemicals. Here, we describe detailed protocols for the quantitative and qualitative assessment of chemotaxis responses that are categorized into short-term direct population response assays and long-term metabolism-based assays that can be used to identify novel chemoeffector molecules and the specific chemoreceptors involved. We emphasize the importance of behavior-based assays to verify the biochemical and physiological relevance of newly identified chemoeffector-receptor pairs.
尽管细菌趋化性的机制已在肠道细菌中得到广泛研究，但寻找新型和非典型化学效应物（无论是在肠道细菌还是远缘相关物种中）需要对经典趋化性测定方法进行改进，以处理难处理且可能有毒的化学物质。在此，我们描述了用于趋化性反应定量和定性评估的详细方案，这些方案分为短期直接群体反应测定和基于长期代谢的测定，可用于鉴定新型化学效应物分子和相关的特定化学感受器。我们强调基于行为的测定对于验证新鉴定的化学效应物 - 受体对的生化和生理相关性的重要性。
