Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea.
Center for Bioanalysis, Korea Research Institute of Standards and Science, Daejeon 34113, Korea.
Mol Cells. 2018 Feb 28;41(2):150-159. doi: 10.14348/molcells.2018.2305. Epub 2018 Feb 12.
Animals use their odorant receptors to receive chemical information from the environment. Insect odorant receptors differ from the G protein-coupled odorant receptors in vertebrates and nematodes, and very little is known about their protein-protein interactions. Here, we introduce a mass spectrometric platform designed for the large-scale analysis of insect odorant receptor protein-protein interactions. Using this platform, we obtained the first Orco interactome from . From a total of 1,186 identified proteins, we narrowed the interaction candidates to 226, of which only two-thirds have been named. These candidates include the known olfactory proteins Or92a and Obp51a. Around 90% of the proteins having published names likely function inside the cell, and nearly half of these intracellular proteins are associated with the endomembrane system. In a basic loss-of-function electrophysiological screen, we found that the disruption of eight (i.e., Rab5, CG32795, Mpcp, Tom70, Vir-1, CG30427, Eaat1, and CG2781) of 28 randomly selected candidates affects olfactory responses . Thus, because this Orco interactome includes physiologically meaningful candidates, we anticipate that our platform will help guide further research on the molecular mechanisms of the insect odorant receptor family.
动物利用其气味受体从环境中接收化学信息。昆虫气味受体与脊椎动物和线虫中的 G 蛋白偶联气味受体不同，关于它们的蛋白质-蛋白质相互作用，人们知之甚少。在这里，我们介绍了一个专为大规模分析昆虫气味受体蛋白-蛋白相互作用而设计的质谱平台。使用该平台，我们从 中获得了第一个 Orco 相互作用组。在总共鉴定出的 1186 种蛋白质中，我们将相互作用候选物缩小到 226 种，其中只有三分之二有名称。这些候选物包括已知的嗅觉蛋白 Or92a 和 Obp51a。约 90%有已发表名称的蛋白质可能在细胞内发挥作用，而这些细胞内蛋白质中有近一半与内膜系统有关。在一个基本的功能丧失型电生理学筛选中，我们发现 28 个随机选择的候选物中的 8 个（即 Rab5、CG32795、Mpcp、Tom70、Vir-1、CG30427、Eaat1 和 CG2781）的破坏会影响嗅觉反应。因此，由于这个 Orco 相互作用组包含有生理意义的候选物，我们预计我们的平台将有助于指导对昆虫气味受体家族的分子机制的进一步研究。
