Zhao Yunpo, Li Haichao, Miao Xuexia
Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, People's Republic of China.
J Chem Ecol. 2015 Nov;41(11):1037-42. doi: 10.1007/s10886-015-0643-1. Epub 2015 Oct 29.
The silkworm Bombyx mori is an oligophagous insect that feeds mainly on mulberry leaves. The olfactory system of silkworm is a good model to study olfaction in Lepidoptera. Here, we carried out shotgun proteomic analysis and MS sequencing of the silkmoth antennae. A total of 364 proteins were detected, 77 were female specific, 143 were male specific, and 144 were expressed in both male and female antennae. Five odorant-binding proteins, two chemosensory proteins, and one olfactory receptor were identified. They may play a major role in the perception of odorants. An esterase and an aldehyde dehydrogenase were found only in male antennae. Glutathione S-transferases (GSTs) and cytochrome P450s, also found in silkworm antennae, may be involved in the degradation of xenobiotics. Additionally, antioxidation proteins and immunity proteins were identified. Juvenile hormone binding proteins (JHBP), juvenile hormone resistance protein II, and juvenile hormone episode hydrolase (JHEH) were found in the proteomic analysis, which suggests that the antennae are a target for juvenile hormone in the silkworm. Our results provide insight into the expression of proteins in the antennae of silkworm and will facilitate the future functional analysis of silkworm antennae.
家蚕是一种寡食性昆虫，主要以桑叶为食。家蚕的嗅觉系统是研究鳞翅目昆虫嗅觉的良好模型。在此，我们对家蚕蛾触角进行了鸟枪法蛋白质组分析和质谱测序。共检测到364种蛋白质，其中77种为雌性特异蛋白，143种为雄性特异蛋白，144种在雌雄触角中均有表达。鉴定出5种气味结合蛋白、2种化学感受蛋白和1种嗅觉受体。它们可能在气味感知中起主要作用。仅在雄性触角中发现一种酯酶和一种醛脱氢酶。在家蚕触角中还发现的谷胱甘肽S-转移酶（GSTs）和细胞色素P450s可能参与外源化合物的降解。此外，还鉴定出了抗氧化蛋白和免疫蛋白。在蛋白质组分析中发现了保幼激素结合蛋白（JHBP）、保幼激素抗性蛋白II和保幼激素酯水解酶（JHEH），这表明触角是家蚕中保幼激素的作用靶点。我们的结果为了解家蚕触角中蛋白质的表达提供了见解，并将有助于未来对家蚕触角的功能分析。
