Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering , Kyoto University , Katsura, Nishikyo-ku, Kyoto 615-8510 , Japan.
CREST(Core Research for Evolutional Science and Technology, JST) , Sanbancho, Chiyodaku, Tokyo , 102-0075 , Japan.
ACS Sens. 2018 Mar 23;3(3):527-539. doi: 10.1021/acssensors.7b00894. Epub 2018 Feb 15.
Chemically constructed biosensors consisting of a protein scaffold and an artificial small molecule have recently been recognized as attractive analytical tools for the specific detection and real-time monitoring of various biological substances or events in cells. Conventionally, such semisynthetic biosensors have been prepared in test tubes and then introduced into cells using invasive methods. With the impressive advances seen in bioorthogonal protein conjugation methodologies, however, it is now becoming feasible to directly construct semisynthetic biosensors in living cells, providing unprecedented tools for life-science research. We discuss here recent efforts regarding the in situ construction of protein-based semisynthetic biosensors and highlight their uses in the visualization and quantification of biomolecules and events in multimolecular and crowded cellular systems.
近年来，由蛋白质支架和人工小分子组成的化学构建生物传感器已被认为是用于特定检测和实时监测细胞内各种生物物质或事件的有吸引力的分析工具。传统上，此类半合成生物传感器是在试管中制备的，然后使用侵入性方法引入细胞。然而，随着生物正交蛋白质偶联方法的显著进展，现在已经可以在活细胞中直接构建半合成生物传感器，为生命科学研究提供了前所未有的工具。我们在这里讨论了关于原位构建基于蛋白质的半合成生物传感器的最新进展，并强调了它们在多分子和拥挤细胞系统中生物分子和事件的可视化和定量中的应用。
