National Research Laboratory for Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea, Seoul 06591, Korea.
Avixgen Inc., Seoul 06649, Korea.
BMB Rep. 2018 Jun;51(6):290-295. doi: 10.5483/bmbrep.2018.51.6.231.
Y-box binding protein 1 (YB-1) is a member of the cold-shock domain (CSD) protein superfamily. It participates in a wide variety of cellular events, including transcription, RNA splicing, translation, DNA repair, drug resistance, and stress responses. We investigated putative functions of YB-1 in HIV-1 replication. Functional studies using overexpression or knockdown of YB-1 in conjunction with transfection of proviral DNA showed that YB-1 enhances virus production. We found YB-1 regulates HIV-1 production by stimulating viral transcription using HIV-1 LTR sequence U3RU5 with Luciferase assay. We also identified a specific region from amino acids 1 to 324 of YB-1 as necessary for the participation of the protein in the production of virions. [BMB Reports 2018; 51(6): 290-295].
Y 盒结合蛋白 1（YB-1）是冷休克结构域（CSD）蛋白超家族的一员。它参与了广泛的细胞事件，包括转录、RNA 剪接、翻译、DNA 修复、耐药性和应激反应。我们研究了 YB-1 在 HIV-1 复制中的可能作用。通过转染前病毒 DNA 并过表达或敲低 YB-1 进行的功能研究表明，YB-1 增强了病毒的产生。我们发现 YB-1 通过使用 Luciferase 测定法从 HIV-1 LTR 序列 U3RU5 刺激病毒转录来调节 HIV-1 的产生。我们还鉴定了 YB-1 中从氨基酸 1 到 324 的特定区域，该区域对于该蛋白参与病毒粒子的产生是必需的。[BMB 报告 2018；51(6): 290-295]。
