Preliminary molecular detection of the somatic embryogenesis receptor-like kinase () and knotted-like homeobox () genes during in vitro morphogenesis of Jacks.

This work aimed to evaluate the embryogenic competence of different tissues from different stages (friable callus, bud-regenerating callus, and whole buds) of , through the molecular detection of the somatic embryogenesis receptor-like kinase () and knotted-like homeobox () genes. RNA was extracted with Trizol, cDNA was obtained, and the studied transcripts were amplified. Using non-specific primers, and gene expression was detected in the three stages evaluated. This study might contribute to providing an explanation for the recalcitrance of this species to somatic embryogenesis.