Ramírez-Mosqueda Marco A, Iglesias-Andreu Lourdes G, Sáenz Luis, Córdova Iván
1Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Av. de las Culturas Veracruzanas No. 101, Campus para la Cultura, las Artes y el Deporte, Col. Emiliano Zapata, 91090 Xalapa, Veracruz Mexico.
2Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Colonia Chuburná de Hidalgo, Mérida, Yucatán Mexico.
3 Biotech. 2018 Feb;8(2):94. doi: 10.1007/s13205-018-1112-7. Epub 2018 Jan 24.
This work aimed to evaluate the embryogenic competence of different tissues from different stages (friable callus, bud-regenerating callus, and whole buds) of , through the molecular detection of the somatic embryogenesis receptor-like kinase () and knotted-like homeobox () genes. RNA was extracted with Trizol, cDNA was obtained, and the studied transcripts were amplified. Using non-specific primers, and gene expression was detected in the three stages evaluated. This study might contribute to providing an explanation for the recalcitrance of this species to somatic embryogenesis.
这项工作旨在通过对体细胞胚胎发生受体样激酶（SERK）和类knotted同源框（KNOX）基因的分子检测，评估不同阶段（易碎愈伤组织、芽再生愈伤组织和完整芽）的不同组织的胚性能力。用Trizol提取RNA，获得cDNA，并扩增所研究的转录本。使用非特异性引物，在评估的三个阶段检测SERK和KNOX基因表达。本研究可能有助于解释该物种对体细胞胚胎发生的顽拗性。
