表征基因组整合融合体中绿色荧光蛋白（GFP）和单体红色荧光蛋白（mCherry）的成熟情况 。 需注意，你提供的原文似乎不完整，“in.”后面应该还有具体内容。
Characterising Maturation of GFP and mCherry of Genomically Integrated Fusions in .
作者信息
Shashkova Sviatlana, Wollman Adam Jm, Hohmann Stefan, Leake Mark C
机构信息
Biological Physical Science Institute, Departments of Physics and Biology, University of York, York, UK.
Department of Chemistry and Molecular Biology, University of Gothenburg, Göteborg, Sweden.
出版信息
Bio Protoc. 2018 Jan 20;8(2):e2710. doi: 10.21769/BioProtoc.2710.
Abstract
Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters. Fluorescent proteins must undergo maturation after expression before they become photoactive. Maturation effects must be quantified during single-molecule analysis. Here we present a method to characterise maturation of GFP and mCherry genetic protein fusions in budding yeast .
摘要
单分子荧光显微镜能够对天然蛋白质与荧光蛋白报告基因的基因组编码融合体进行无与伦比的亚细胞定量分析。荧光蛋白在表达后必须经历成熟过程才能变得具有光活性。在单分子分析过程中必须对成熟效应进行量化。在此，我们提出一种在芽殖酵母中表征绿色荧光蛋白（GFP）和单体红色荧光蛋白（mCherry）基因蛋白融合体成熟过程的方法。
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本文引用的文献
1
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FEMS Microbiol Lett. 2017 Aug 1;364(14). doi: 10.1093/femsle/fnx133.
2
Transcription factor clusters regulate genes in eukaryotic cells.转录因子簇调节真核细胞中的基因。
Elife. 2017 Aug 25;6:e27451. doi: 10.7554/eLife.27451.
3
Frequent exchange of the DNA polymerase during bacterial chromosome replication.细菌染色体复制过程中DNA聚合酶的频繁交换。
Elife. 2017 Mar 31;6:e21763. doi: 10.7554/eLife.21763.
4
Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time.毫秒级单分子定位显微镜结合卷积分析和自动图像分割技术，每次对一个细胞进行分析，以确定复杂结构的功能细胞中的蛋白质浓度。
Faraday Discuss. 2015;184:401-24. doi: 10.1039/c5fd00077g. Epub 2015 Sep 30.
5
The physics of life: one molecule at a time.生命物理学：一次一个分子。
Philos Trans R Soc Lond B Biol Sci. 2012 Dec 24;368(1611):20120248. doi: 10.1098/rstb.2012.0248. Print 2013 Feb 5.
6
In vivo architecture and action of bacterial structural maintenance of chromosome proteins.细菌染色体结构维持蛋白的体内结构和作用。
Science. 2012 Oct 26;338(6106):528-31. doi: 10.1126/science.1227126.
7
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Nat Biotechnol. 2012 Jun 24;30(7):708-14. doi: 10.1038/nbt.2281.
8
Stoichiometry and turnover in single, functioning membrane protein complexes.单一功能性膜蛋白复合物中的化学计量与周转率。
Nature. 2006 Sep 21;443(7109):355-8. doi: 10.1038/nature05135. Epub 2006 Sep 13.
9
Genetic control of the cell-division cycle in yeast. I. Detection of mutants.酵母细胞分裂周期的遗传控制。I. 突变体的检测。
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