Oishi K, Yamamoto M, Yoshida T, Ide M, Matsumoto K
Tohoku J Exp Med. 1986 May;149(1):95-102. doi: 10.1620/tjem.149.95.
The binding of 125I-fibronectin (FN) to some bacteria and the phagocytic activity of human alveolar macrophages (AM) by a culture method using 3H-labelled bacteria were examined. FN-binding of Staphylococcus aureus (S. aureus) was higher than that of gram-negative rods and was reduced markedly by trypsin-treatment. FN enhanced the uptake of S. aureus by AM in dose dependent manner. To determine whether FN-binding to S. aureus mediates the promotion of staphylococcal phagocytosis, we compared the uptake of non-treated S. aureus with that of trypsin-treated bacteria by AM. No significant difference was found between the uptake of these bacteria and no effect of FN-binding to S. aureus on the phagocytic activity. It is concluded that FN plays an essential role as a non-immune opsonin for S. aureus in the human lung which is found to be abundant in FN, and that FN-binding to S. aureus is probably not related to this effect.
采用³H标记细菌的培养方法，检测了¹²⁵I-纤连蛋白（FN）与某些细菌的结合以及人肺泡巨噬细胞（AM）的吞噬活性。金黄色葡萄球菌（S. aureus）的FN结合高于革兰氏阴性杆菌，且经胰蛋白酶处理后显著降低。FN以剂量依赖方式增强AM对金黄色葡萄球菌的摄取。为了确定FN与金黄色葡萄球菌的结合是否介导了葡萄球菌吞噬作用的促进，我们比较了AM对未处理的金黄色葡萄球菌和经胰蛋白酶处理的细菌的摄取情况。这些细菌的摄取之间未发现显著差异，且FN与金黄色葡萄球菌的结合对吞噬活性无影响。结论是，FN作为人肺中富含FN的金黄色葡萄球菌的非免疫调理素发挥着重要作用，并且FN与金黄色葡萄球菌的结合可能与这种作用无关。
