Department of Anatomy and Cell Biology, McGill University, Montréal, Québec, Canada H3A 0C7.
Groupe de Recherche Axé sur la Structure des Protéines (GRASP), Montréal, Québec, Canada H3A 0C7.
Bioessays. 2018 Mar;40(3). doi: 10.1002/bies.201700209. Epub 2018 Feb 12.
Motile eukaryotic cilia and flagella are hair-like organelles responsible for cell motility and mucociliary clearance. Using cryo-electron tomography, it has been shown that the doublet microtubule, the cytoskeleton core of the cilia and flagella, has microtubule inner protein structures binding periodically inside its lumen. More recently, single-particle cryo-electron microscopy analyses of isolated doublet microtubules have shown that microtubule inner proteins form a meshwork inside the doublet microtubule. High-resolution structures revealed new types of interactions between the microtubule inner proteins and the tubulin lattice. In addition, they offered insights into the potential roles of microtubule inner proteins in the stabilization and assembly of the doublet microtubule. Herein, we review our new insights into microtubule inner proteins from the doublet microtubule together with the current body of literature on microtubule inner proteins.
游动的真核纤毛和鞭毛是负责细胞运动和黏液纤毛清除的毛发状细胞器。使用冷冻电子断层扫描技术已经表明，纤毛和鞭毛的细胞骨架核心——双联体微管在其管腔内部有周期性结合的微管内蛋白结构。最近，对分离的双联体微管进行的单颗粒冷冻电子显微镜分析表明，微管内蛋白在双联体微管内形成一个网格。高分辨率结构揭示了微管内蛋白与微管晶格之间的新型相互作用。此外，它们还深入了解了微管内蛋白在双联体微管的稳定和组装中的潜在作用。本文中，我们综述了从双联体微管中获得的关于微管内蛋白的新见解，并结合了当前关于微管内蛋白的文献。
