Gaur Tanvi, Rao P B
Department of Biological Sciences, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India.
Department of Biological Sciences, College of Basic Sciences & Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar 263145 (U.S. Nagar, Uttarakhand), India.
Int J Med Mushrooms. 2017;19(12):1083-1092. doi: 10.1615/IntJMedMushrooms.2017024559.
The antibacterial activity, phenolic profile, and bioactive compounds of fruiting bodies from 2 strains (MA1 and MA2) of the giant mushroom Macrocybe gigantea were evaluated to access their nutraceutical efficacy. The antibacterial activity was higher in MA2 against all selected pathogenic bacteria. Selected phenolics were analyzed by high-performance liquid chromatography coupled with ultraviolet-visible detection. Gallic acid, ferulic acid, quercetin, p-hydroxy benzoic acid, cinnamic acid, and rutin contents (micrograms per gram dry weight) were quantified. Quercetin and rutin were absent in both strains of M. gigantea. M. gigantea MA2 showed relatively higher phenolic content (915.8 μg/g dry weight) than M. gigantea MA1 (854.4 μg/g dry weight). Among the phenolics, gallic acid is found in the largest amount; in M. gigantea MA2, it was 847.9 ± 2.67 μg/g dry weight. Gas chromatography-mass spectrometry analysis showed the presence of bioactive compounds in both strains; most compounds were antibacterial. Thus, the selected strains of M. gigantea can combat oxidative damage and can be used in foods, pharmaceuticals, and cosmetics because of their antioxidant potential.
对巨大口蘑（Macrocybe gigantea）的2个菌株（MA1和MA2）子实体的抗菌活性、酚类成分和生物活性化合物进行了评估，以确定它们的营养保健功效。MA2对所有选定的病原菌的抗菌活性更高。通过高效液相色谱结合紫外可见检测对选定的酚类物质进行了分析。对没食子酸、阿魏酸、槲皮素、对羟基苯甲酸、肉桂酸和芦丁含量（微克/克干重）进行了定量。巨大口蘑的两个菌株中均未检测到槲皮素和芦丁。巨大口蘑MA2的酚类含量（915.8微克/克干重）相对高于巨大口蘑MA1（854.4微克/克干重）。在酚类物质中，没食子酸含量最高；在巨大口蘑MA2中，其含量为847.9±2.67微克/克干重。气相色谱-质谱分析表明两个菌株中均存在生物活性化合物；大多数化合物具有抗菌作用。因此，选定的巨大口蘑菌株可以对抗氧化损伤，由于其抗氧化潜力，可用于食品、药品和化妆品。
