Wink C S
Acta Anat (Basel). 1986;126(1):57-62. doi: 10.1159/000146187.
This study compared SEM appearances of endosteal and periosteal surfaces of anorganic femoral diaphyses from three groups of adult male rats: (1) control; (2) castrate (osteoporotic), and (3) castrate + injections of dichloromethylene biphosphonate (Cl2 entrances; (2) area of vascular canal entrances; (3) number of osteoblast lacunae, and (4) percent area of bone surface types. Endosteal surfaces from untreated osteoporotic rats had significantly more osteoblast lacunae, larger vascular canal entrances, and less resting surface than those of the other groups. There were no differences in endosteal surfaces between controls and Cl2MBP-treated castrates. Periosteal surfaces demonstrated no significant differences in percent area of bone surface types between groups, untreated castrates had more osteoblast lacunae than other groups, and vascular canal entrances were smaller in castrate groups than in controls. Castrates treated with Cl2MBP had significantly fewer periosteal vascular canal entrances, and many canal entrances and osteoblast lacunae appeared to be plugged with mineral deposits when compared with the other groups. The results indicate that Cl2MBP treatment prevented endosteal bone surface changes that occurred with castration osteoporosis, and may have disrupted normal vascularization on periosteal surfaces.
（1）对照组；（2）去势（骨质疏松）组，以及（3）去势+注射二氯亚甲基二膦酸盐（Cl2MBP）组。观察指标包括：（1）血管通道入口面积；（2）血管通道入口面积；（3）成骨细胞陷窝数量，以及（4）骨表面类型的面积百分比。未经治疗的骨质疏松大鼠的骨内膜表面比其他组具有明显更多的成骨细胞陷窝、更大的血管通道入口和更少的静止表面。对照组和Cl2MBP治疗的去势大鼠的骨内膜表面没有差异。骨膜表面在各组之间的骨表面类型面积百分比上没有显著差异，未经治疗的去势大鼠比其他组有更多的成骨细胞陷窝，去势组的血管通道入口比对照组小。与其他组相比，用Cl2MBP治疗的去势大鼠的骨膜血管通道入口明显更少，并且许多通道入口和成骨细胞陷窝似乎被矿物质沉积物堵塞。结果表明，Cl2MBP治疗可预防去势骨质疏松时发生的骨内膜骨表面变化，并可能破坏骨膜表面的正常血管化。
