最多等待 10 秒，若超时请稍后重试。
Liru Dou, KaiKai He, Takumi Higaki, Xiangfeng Wang, Tonglin Mao
State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
International Research Organization for Advanced Science and Technology, Kumamoto University, Kurokami, Chuou-ku, Kumamoto 860-8555, Japan.
Plant Physiol. 2018 Mar;176(3):2071-2081. doi: 10.1104/pp.17.01124. Epub 2018 Feb 5.
Regulation of cortical microtubule reorganization is essential for plant cell survival under high salinity conditions. In response to salt stress, microtubules undergo rapid depolymerization followed by reassembly to form a new microtubule network that promotes cell survival; however, the upstream regulatory mechanisms for this recovery response are largely unknown. In this study, we demonstrate that ethylene signaling facilitates salt stress-induced reassembly of cortical microtubules in Arabidopsis (). Microtubule depolymerization was not affected under salt stress following the suppression of ethylene signaling with Ag or in ethylene-insensitive mutants, whereas microtubule reassembly was significantly inhibited. ETHYLENE-INSENSITIVE3, a key transcription factor in the ethylene signaling pathway, was shown to play a central role in microtubule reassembly under salt stress. In addition, we performed functional characterization of the microtubule-stabilizing protein WAVE-DAMPENED2-LIKE5 (WDL5), which was found to promote ethylene-associated microtubule reassembly and plant salt stress tolerance. These findings indicate that ethylene signaling regulates microtubule reassembly by up-regulating expression in response to salt stress, thereby implicating ethylene signaling in salt-stress tolerance in plants.
皮层微管重组的调控对于植物细胞在高盐条件下的存活至关重要。在盐胁迫下，微管迅速解聚，然后重新组装形成新的微管网络，促进细胞存活；然而，这种恢复反应的上游调控机制在很大程度上尚不清楚。在这项研究中，我们证明了乙烯信号促进了拟南芥中盐胁迫诱导的皮层微管重组。在 Ag 处理或乙烯不敏感突变体中抑制乙烯信号后，盐胁迫下微管的解聚不受影响，而微管的重组则受到显著抑制。ETHYLENE-INSENSITIVE3 是乙烯信号通路中的关键转录因子，在盐胁迫下的微管重组中发挥核心作用。此外，我们对微管稳定蛋白 WAVE-DAMPENED2-LIKE5 (WDL5) 的功能进行了表征，发现它能促进与乙烯相关的微管重组和植物耐盐性。这些发现表明，乙烯信号通过响应盐胁迫上调表达来调节微管的重组，从而表明乙烯信号参与了植物的耐盐性。
