Danieli Betto D, Zerbato E, Betto R
Biochem Biophys Res Commun. 1986 Jul 31;138(2):981-7. doi: 10.1016/s0006-291x(86)80592-7.
Mammalian skeletal muscles are mixture of three type of fibers: type 1, type 2A, and type 2B fibers. Immunological studies and proteolytic analysis of myosin heavy chains from the three type of fibers have demonstrated the presence of distinct myosin isoforms. By using typed single muscle fibers and improving an electrophoretic method we are able to resolve three distinct polypeptides which are demonstrate to correspond to type 1, 2A and 2B myosin heavy chain isoforms by using specific monoclonal antibodies. The analysis of single muscle fibers shows that different myosin heavy chain isoforms are frequently coexpressed in the same muscle fiber.
1型、2A型和2B型纤维。对这三种纤维的肌球蛋白重链进行的免疫学研究和蛋白水解分析表明存在不同的肌球蛋白同工型。通过使用分型的单根肌纤维并改进电泳方法，我们能够分离出三种不同的多肽，通过使用特异性单克隆抗体证明它们分别对应于1型、2A型和2B型肌球蛋白重链同工型。对单根肌纤维的分析表明，不同的肌球蛋白重链同工型经常在同一肌纤维中共表达。
