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Selective precipitation of phosphofructokinase, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase from rat erythrocyte hemolysates by polyethylene glycol.

作者信息

Tejedor M C, Delgado C, Rubio A, Luque J

出版信息

Biosci Rep. 1986 Apr;6(4):395-401. doi: 10.1007/BF01116427.

DOI:10.1007/BF01116427
PMID:2943327
Abstract

Precipitation profiles of phosphofructokinase, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase have been established in the range of 0-16% PEG at different pH values. Precipitation generally occurred between narrow limits of polyethylene glycol. The polymer concentration needed to reach any level of enzyme precipitation is dependent on pH. Particular conditions (% PEG and pH) for the selective enzyme enrichment have been determined.

摘要

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