* Department of Applied Life Science, Graduate School, Konkuk University, Chungju, Korea.
† Department of Food Science and Engineering, Seowon University, Cheongju, Korea.
Am J Chin Med. 2018;46(2):423-433. doi: 10.1142/S0192415X18500210. Epub 2018 Feb 12.
Eucalyptus globulus Labill. (E. globulus, Myrtaceae) is used in Europe as a traditional folk remedy for inflammation-related disorders such as arthritis, diabetes, asthma, and gout. We investigated this study to evaluate the protective effects of E. globulus extract (EG) on inflammatory responses, and provide scientific and mechanistic evidence in in vitro and in vivo experimental models. LPS-stimulated murine bone marrow-derived macrophages (BMDMs) were used to study the regulatory effect of EG on inflammasome activation in vitro. Monosodium urate (MSU)-induced peritonitis was used to study the effect of EG in an in vivo murine model. EG suppressed IL-[Formula: see text] secretion via the regulation of apoptosis-associated speck-like proteins containing a CARD (ASC) oligomerization and caspase-1 maturation, leading to the inhibition of inflammasome activation. In the in vivo study, EG suppressed the MSU-induced peritonitis by attenuating interleukin (IL)-1[Formula: see text], providing scientific support for its traditional use in the treatment of inflammation-related disorders.
蓝桉（Eucalyptus globulus Labill.，桃金娘科）在欧洲被用作传统民间疗法，用于治疗与炎症相关的疾病，如关节炎、糖尿病、哮喘和痛风。本研究旨在评估蓝桉提取物（EG）对炎症反应的保护作用，并提供体外和体内实验模型的科学和机制证据。我们使用脂多糖（LPS）刺激的鼠骨髓来源的巨噬细胞（BMDMs）来研究 EG 对炎症小体激活的调节作用。我们使用尿酸单钠（MSU）诱导的腹膜炎来研究 EG 在体内鼠模型中的作用。EG 通过调节凋亡相关斑点样蛋白包含一个 CARD（ASC）寡聚化和半胱氨酸天冬氨酸蛋白酶-1（caspase-1）成熟，抑制炎症小体激活，从而抑制白细胞介素（IL）-1β的分泌。在体内研究中，EG 通过抑制白细胞介素（IL）-1β来抑制 MSU 诱导的腹膜炎，为其在治疗与炎症相关的疾病中的传统应用提供了科学支持。
