Fujii Yosuke, Ding Yuqi, Umezawa Taichi, Akimoto Takafumi, Xu Jiawei, Uchida Takashi, Fujino Tatsuya
Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University.
Department of Food Inspection, Zhejiang Institute for Food and Drug Control.
Anal Sci. 2018;34(2):221-225. doi: 10.2116/analsci.34.221.
Food additives generally used in carbonated drinks, such as 4-methylimidazole (4MI), caffeine (Caf?), citric acid (CA), and aspartame (Apm), were measured by matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) using nanometer-sized particles of iron oxide (FeO NPs). The quantification of 4MI in Coca Cola (C-cola) was carried out. In order to improve the reproducibility of the peak intensities, FeO NPs loaded on ZSM5 zeolite were used as the matrix for quantification. By using 2-ethylimidazole (2EI) as the internal standard, the amount of 4MI in C-cola was determined to range from 88 to 65 μg/355 mL. The results agree with the published value (approx. 72 μg/355 mL). It was found that MALDI using FeO was applicable to the quantification of 4MI in C-cola.
使用纳米级氧化铁颗粒（FeO NPs）通过基质辅助激光解吸电离质谱法（MALDI MS）测定了碳酸饮料中常用的食品添加剂，如4-甲基咪唑（4MI）、咖啡因（Caf?）、柠檬酸（CA）和阿斯巴甜（Apm）。对可口可乐（C-可乐）中的4MI进行了定量分析。为了提高峰强度的重现性，将负载在ZSM5沸石上的FeO NPs用作定量分析的基质。以2-乙基咪唑（2EI）为内标，测定出C-可乐中4MI的含量范围为88至65μg/355 mL。该结果与已发表的值（约72μg/355 mL）相符。研究发现，使用FeO的MALDI适用于C-可乐中4MI的定量分析。
