一株源自温泉的新菌株（PFAB2）分泌的胞外多糖的结构与功能特性
Structural and Functional Properties of Exopolysaccharide Excreted by a Novel (Strain PFAB2) of Hot Spring Origin.
作者信息
Banerjee Aparna, Rudra Shalini Gaur, Mazumder Koushik, Nigam Vinod, Bandopadhyay Rajib
机构信息
1UGC-CAS, Department of Botany, The University of Burdwan, Golapbag, Burdwan, West Bengal 713104 India.
2Division of Food Science and Postharvest Technology, ICAR-Indian Agricultural Research Institute, Pusa, New Delhi, 110012 India.
出版信息
Indian J Microbiol. 2018 Mar;58(1):39-50. doi: 10.1007/s12088-017-0699-4. Epub 2017 Dec 29.
Abstract
Exopolysaccharide produced by a unique avirulent strain PFAB2 of hot spring origin has been characterized and its functional properties are investigated which is a first report. Maximum yield of EPS is 7.66 g/l with 2% glucose and 1% peptone as optimum carbon and nitrogen source respectively. The EPS is found to be a homopolymer consisting of only glucose as principle monosaccharide component. Through H NMR study, different dextran-like proton peaks are observed. Molecular weight of the EPS resembles low molecular weight bacterial origin polysaccharides. Melting transition of the EPS has started after 276 °C which indicates good thermal stability. The EPS also shows potent antioxidant activity in terms of DPPH and ABTS mediated free radical scavenging property compared to standard ascorbic acid. Emulsifying property of the EPS is also observed and has shown good emulsification of vegetable oils. The polysaccharide forms a thermo resistant gel during the heating phase, with G' higher than G″ indicating excellent shear-thinning behaviour and viscoelastic nature of the EPS.
摘要
温泉源无毒独特菌株PFAB2产生的胞外多糖已得到表征，并对其功能特性进行了研究，这是首次报道。以2%葡萄糖和1%蛋白胨分别作为最佳碳源和氮源时，胞外多糖的最大产量为7.66 g/l。发现该胞外多糖是一种均聚物，仅以葡萄糖作为主要单糖成分。通过核磁共振氢谱研究，观察到不同的类葡聚糖质子峰。该胞外多糖的分子量类似于低分子量细菌来源的多糖。胞外多糖在276°C后开始发生熔融转变，这表明其具有良好的热稳定性。与标准抗坏血酸相比，该胞外多糖在DPPH和ABTS介导的自由基清除性能方面也表现出强大的抗氧化活性。还观察到该胞外多糖的乳化性能，并且它对植物油表现出良好的乳化作用。该多糖在加热阶段形成耐热凝胶，储能模量高于损耗模量，表明该胞外多糖具有优异的剪切变稀行为和粘弹性。
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