Wang Zhifa, Xing Hongyan, Hu Hanqing, Dai Taiqiang, Wang Yan, Li Zhijin, An Ran, Xu Haiyan, Liu Yanpu, Liu Bin
Department of Stomatology, Guangzhou General Hospital of Guangzhou Command, Guangzhou, Guangdong 510010, P.R. China.
Department of Oral and Maxillofacial Surgery, State Key Laboratory of Military Stomatology, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
Exp Ther Med. 2018 Jan;15(1):795-805. doi: 10.3892/etm.2017.5497. Epub 2017 Nov 13.
The aim of the present study was to determine the effect of adipose-derived mesenchymal stem cells (ADSCs) combined with heterologous platelet-rich fibrin extract (PRFe) on irradiation-induced salivary gland (SG) damage. ADSCs were isolated from C3H mice, whereas PRFe was obtained from New Zealand rabbits. Twelve weeks post irradiation, the ADSCs or PRFe or their combination were transplanted into the submandibular glands of C3H mice with irradiation-induced damage. The salivary flow rate (SFR) was determined and histopathological analysis was performed at 12 weeks post transplantation. Immunofluorescence, microvessel density measurements and transmission electron microscopy were performed to assess α-amylase (AMY) production, apoptosis and microstructural changes in the cells. The administration of ADSCs combined with PRFe increased the SFR at 12 weeks post transplantation, whereas ADSCs alone or PRFe alone failed to do so. The ADSCs+PRFe-treated, irradiated SGs had fewer damaged and atrophied acinar cells, higher AMY levels and an increased microvessel density compared with the untreated irradiated SGs. Moreover, SG tissue from the ADSCs+PRFe group also showed decreased apoptotic and increased proliferative activity compared to that from the irradiated group. In conclusion, ADSCs or PRFe alone did not restore permanent, irradiation-induced damage of SG tissue when used alone, but when used together, they provided effective treatment outcomes.
本研究的目的是确定脂肪来源的间充质干细胞（ADSCs）与异源富血小板纤维蛋白提取物（PRFe）联合应用对辐射诱导的唾液腺（SG）损伤的影响。ADSCs从C3H小鼠中分离，而PRFe从新西兰兔中获得。照射后12周，将ADSCs或PRFe或它们的组合移植到有辐射诱导损伤的C3H小鼠的下颌下腺中。在移植后12周测定唾液流速（SFR）并进行组织病理学分析。进行免疫荧光、微血管密度测量和透射电子显微镜检查以评估α-淀粉酶（AMY）的产生、细胞凋亡和微观结构变化。ADSCs与PRFe联合给药在移植后12周时增加了SFR，而单独使用ADSCs或单独使用PRFe则未能如此。与未治疗的受照射唾液腺相比，ADSCs+PRFe处理的受照射唾液腺中受损和萎缩的腺泡细胞更少，AMY水平更高，微血管密度增加。此外，与照射组相比，ADSCs+PRFe组的唾液腺组织还显示凋亡减少，增殖活性增加。总之，单独使用ADSCs或PRFe时，不能恢复辐射诱导的唾液腺组织永久性损伤，但联合使用时，它们提供了有效的治疗效果。
