Jin Feng, Zhu Guang, Li Dan, Ni Tengyang, Dai Xiaojun, Wang Haibo, Feng Jun, Qian Yayun, Yang Lin, Guo Shiyu, Hisamitsu Tadashi, Liu Yanqing
Institute of Traditional Chinese Medicine and Western Medicine, School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China.
Department of Oncology, Yangzhou Hospital of Traditional Chinese Medicine, Yangzhou, Jiangsu 225009, P.R. China.
Oncol Lett. 2018 Feb;15(2):1591-1599. doi: 10.3892/ol.2017.7459. Epub 2017 Nov 21.
Recently, ethyl acetate extracts (COE) have been investigated for their anticancer effects on digestive tract tumors. However, the therapeutic effects of COE on esophageal squamous carcinoma cells (ESCC) have not been investigated. In the present study, the effects of COE on the cell cycle and apoptosis of ESCCs were assessed , and it was revealed that COE treatment triggered G/G cell cycle arrest, and induced DNA damage and apoptosis in a dose-dependent manner in ESCC. Activation of the phosphatidylinositol 3-kinase/protein kinase-B/mechanistic target of rapamycin (mTOR) pathway was also suppressed by COE. Additionally, the combined treatment with COE and rapamycin (an mTOR inhibitor) acted synergistically in ECA-109 cells compared with the treatment with COE or rapamycin alone. These findings extend the understanding of the action of COE and suggest that COE has potential as a treatment option for ESCC as a single treatment or in combination.
最近，人们对乙酸乙酯提取物（COE）对消化道肿瘤的抗癌作用进行了研究。然而，COE对食管鳞状细胞癌（ESCC）的治疗效果尚未得到研究。在本研究中，评估了COE对ESCC细胞周期和凋亡的影响，结果显示COE处理引发了G/G细胞周期停滞，并以剂量依赖的方式诱导了ESCC中的DNA损伤和凋亡。COE还抑制了磷脂酰肌醇3激酶/蛋白激酶B/雷帕霉素机制靶点（mTOR）通路的激活。此外，与单独使用COE或雷帕霉素相比，COE与雷帕霉素（一种mTOR抑制剂）联合处理在ECA-109细胞中具有协同作用。这些发现扩展了对COE作用的理解，并表明COE作为单一治疗或联合治疗ESCC具有潜在的治疗选择。
