Zeng Zhaoyang, Zhang Hua, Wang Xin, Liu Kai, Li Tian, Sun Shaobo, Li Hailong
College of Integrated Traditional Chinese and Western Medicine, Gansu University of Chinese Medicine, Lanzhou, Gansu 730000, P.R. China.
Department of Orthopedics, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
Oncol Lett. 2018 Feb;15(2):2679-2685. doi: 10.3892/ol.2017.7609. Epub 2017 Dec 13.
The present study aimed to investigate the potential anticancer effect and mechanisms of salvianolic acid B on osteosarcoma. Salvianolic acid B suppressed osteosarcoma cell proliferation and induced apoptosis in the osteosarcoma MG63 cell line, and activated the expressions of cleaved caspase-3, phosphorylated-tumor protein (p)38 mitogen-activated protein kinase (p-p38 MAPK) and phosphorylated-p53 (p-p53) proteins in the MG63 cells. Additionally, Salvianolic acid B also increased the level of reactive oxygen species (ROS) generation in the MG63 cells. The silencing of p38 expression inhibited the anticancer effect of salvianolic acid B on the levels of cell proliferation, p-p53 protein expression and ROS generation level in the MG63 cells. All these data supported the hypothesis that the anticancer effect of salvianolic acid B includes the suppression of cell proliferation and induces apoptosis in MG63 cells, and that p38 is important in the anticancer effect of salvianolic acid B on osteosarcoma cells due to the direct regulation of ROS generation. These data suggest that salvianolic acid B is important in the proliferation of osteosarcoma cells due to the direct regulation of p38-mediated ROS signaling.
本研究旨在探讨丹酚酸B对骨肉瘤的潜在抗癌作用及其机制。丹酚酸B抑制骨肉瘤细胞增殖并诱导骨肉瘤MG63细胞系凋亡，同时激活MG63细胞中裂解的半胱天冬酶-3、磷酸化肿瘤蛋白（p）38丝裂原活化蛋白激酶（p-p38 MAPK）和磷酸化p53（p-p53）蛋白的表达。此外，丹酚酸B还增加了MG63细胞中活性氧（ROS）的生成水平。p38表达的沉默抑制了丹酚酸B对MG63细胞增殖水平、p-p53蛋白表达和ROS生成水平的抗癌作用。所有这些数据支持了以下假设：丹酚酸B的抗癌作用包括抑制MG63细胞增殖并诱导其凋亡，并且p38在丹酚酸B对骨肉瘤细胞的抗癌作用中很重要，因为它直接调节ROS的生成。这些数据表明，丹酚酸B通过直接调节p38介导的ROS信号传导，在骨肉瘤细胞增殖中起重要作用。
