Simoneau J A, Lortie G, Boulay M R, Thibault M C, Bouchard C
Clin Physiol. 1986 Aug;6(4):347-56. doi: 10.1111/j.1475-097x.1986.tb00240.x.
In order to assess the variability in repeated determination of human muscle fibre type distribution, fibre area and enzyme activity measurements, two biopsies were taken within 10 days in the same vastus lateralis for 12 females and 13 males, and in the right and in the left muscles for 25 other subjects (13 females and 12 males). Within muscle, intraclass reliability coefficients were 0.88, 0.82 and 0.56 for type I, IIa and IIb per cent fibres, respectively, and ranged from 0.74 to 0.82 for fibre areas and from 0.71 to 0.90 for enzyme markers of different metabolic pathways. Correlations between right and left muscle measurements were also high for fibre areas (from 0.85 to 0.91) and enzyme activities (from 0.71 to 0.87), except for phosphofructokinase (r = 0.63). In contrast, the right and left thigh muscle correlation reached 0.67, 0.40 and 0.64 for type I, IIa and IIb fibre distribution, respectively. Thus, the variation in muscle sampling and technical procedures reached about 15% of the total variation (i.e. total differences between subjects) for the proportion of fibre type I and IIa and about 20-25% for fibre areas and enzyme activities. On the other hand, the technical error for the proportion of fibre type I and IIa is about 6-7%. This implies that differences brought about by any experimental treatment on these skeletal muscle characteristics in human studies have to be of a relatively large magnitude before being detectable. On the other hand, fibre areas and enzyme activities measured in single needle biopsy sample, from one of the vastus lateralis muscles, are quite representative of the other vastus lateralis. Similarity in fibre type proportion between right and left vastus lateralis cannot be postulated, however, without investigating both muscles.
为了评估人体肌纤维类型分布、纤维面积和酶活性测量结果重复测定中的变异性，在10天内对12名女性和13名男性的同一股外侧肌进行了两次活检，对另外25名受试者（13名女性和12名男性）的左右两侧肌肉进行了活检。在肌肉内部，I型、IIa型和IIb型纤维百分比的组内可靠性系数分别为0.88、0.82和0.56，纤维面积的可靠性系数在0.74至0.82之间，不同代谢途径的酶标志物的可靠性系数在0.71至0.90之间。左右两侧肌肉测量结果之间的相关性对于纤维面积（0.85至0.91）和酶活性（0.71至0.87）也很高，除了磷酸果糖激酶（r = 0.63）。相比之下，左右大腿肌肉中I型、IIa型和IIb型纤维分布的相关性分别为0.67、0.40和0.64。因此，肌肉采样和技术程序的变异性对于I型和IIa型纤维比例约占总变异性（即受试者之间的总体差异）的15%，对于纤维面积和酶活性约占20 - 25%。另一方面，I型和IIa型纤维比例的技术误差约为6 - 7%。这意味着在人体研究中，任何实验处理对这些骨骼肌特征产生的差异必须达到相对较大的程度才能被检测到。另一方面，从股外侧肌之一的单针活检样本中测量的纤维面积和酶活性，相当能代表另一条股外侧肌。然而，如果不检查双侧肌肉，就不能假定左右股外侧肌之间纤维类型比例相似。
