Jo Michiko, Nakamura Norio, Kakiuchi Nobuko, Komatsu Katsuko, Qui Ming-Hua, Shimotohno Kumiko, Shimotohno Kunitada, Hattori Masao
Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan.
Faculty of Pharmaceutical Sciences, Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, Japan.
J Nat Med. 2006 Jul;60(3):217-224. doi: 10.1007/s11418-006-0041-7. Epub 2006 May 24.
For the purpose of developing novel anti-hepatitis C virus (HCV) agents from natural resources, 93 Yunnan crude drugs were screened for their inhibitory effects on RNA-dependent RNA polymerase (RdRp) of HCV. Although 71 methanol extracts and 50 water extracts inhibited HCV-RdRp by more than 50% at a concentration of 50 μg/ml, the majority of them contained a high percentage of tannins. However, methanol extracts of Plumbago zeylanica (branch), Maytenus fookerii (leaf) and Huashidancha (Y61, branch and leaf), and water extracts of Potentilla griffithii (whole plant) and Salvia yunnanensis (underground part), having IC values of less than 10 μg/ml, showed less than 10% tannin content. In addition, from a methanol extract of Tripterygium hypoglaucum (root bark), demethylzeylasteral was isolated as a strongly inhibitory substance against HCV-RdRp.
为了从自然资源中开发新型抗丙型肝炎病毒（HCV）药物，对93种云南天然药物进行了筛选，考察其对HCV的RNA依赖性RNA聚合酶（RdRp）的抑制作用。虽然71种甲醇提取物和50种水提取物在浓度为50μg/ml时对HCV-RdRp的抑制率超过50%，但其中大多数含有高比例的单宁。然而，白花丹（枝）、福氏美登木（叶）和华氏单茶（Y61，枝和叶）的甲醇提取物，以及绢毛委陵菜（全株）和云南鼠尾草（地下部分）的水提取物，其IC值小于10μg/ml，单宁含量低于10%。此外，从昆明山海棠（根皮）的甲醇提取物中分离出脱甲基泽拉斯醇，它是一种对HCV-RdRp有强烈抑制作用的物质。
