Crosti F, Secchi A, Ferrero E, Falqui L, Inverardi L, Pontiroli A E, Ciboddo G F, Pavoni D, Protti P, Rugarli C
Diabetes. 1986 Sep;35(9):1053-7. doi: 10.2337/diab.35.9.1053.
Impairment of suppressor-cell activity may be important in the pathogenesis and maintenance of insulin-dependent diabetes mellitus (IDDM). In 23 recent-onset IDDM patients, lymphocyte sensitivity in vitro to theophylline was tested both in basal conditions and after improvement of metabolic control. This pharmacologic agent is mainly effective on a lymphocytic subpopulation with phenotypic and functional suppressive features. Peripheral blood lymphocytes from IDDM patients showed a loss of theophylline sensitivity, identified as inhibition of both E-rosette formation and blastogenic response to polyclonal mitogens concanavalin A (ConA) and phytohemagglutinin (PHA). An inverse relationship was demonstrated between the theophylline-induced suppression of ConA blastogenic response and blood glucose and glycosylated hemoglobin levels (P less than .01). Metabolic control seemed to be important even in relation to lymphocyte subpopulation distribution. In IDDM patients we found a significant (P less than .05) reduction of OKT4+ lymphocytes that is correlated with blood glucose and glycosylated hemoglobin levels (P less than .01). The improvement of metabolic control led to recovery of theophylline sensitivity. We suggest a deficiency in a suppressive system that could be involved in IDDM onset and the possible role of metabolic control in the impairment of some immunologic functions reported with this pathologic condition.
抑制性细胞活性受损在胰岛素依赖型糖尿病（IDDM）的发病机制及维持过程中可能具有重要作用。在23例新近发病的IDDM患者中，检测了基础状态下以及代谢控制改善后的淋巴细胞体外对茶碱的敏感性。这种药物主要对具有表型和功能抑制特征的淋巴细胞亚群有效。IDDM患者的外周血淋巴细胞显示出对茶碱敏感性丧失，表现为E花环形成及对多克隆有丝分裂原刀豆球蛋白A（ConA）和植物血凝素（PHA）的增殖反应均受到抑制。茶碱诱导的对ConA增殖反应的抑制与血糖及糖化血红蛋白水平之间呈负相关（P<0.01）。代谢控制似乎即使与淋巴细胞亚群分布相关时也很重要。在IDDM患者中，我们发现OKT4 +淋巴细胞显著减少（P<0.05），且与血糖及糖化血红蛋白水平相关（P<0.01）。代谢控制的改善导致茶碱敏感性恢复。我们认为存在一种可能参与IDDM发病的抑制系统缺陷，以及代谢控制在这种病理状态下所报道的某些免疫功能损害中可能发挥的作用。
