Zocchi M R, Pardi R, Gromo G, Ferrero E, Ferrero M E, Besana C, Rugarli C
J Immunopharmacol. 1985;7(2):217-34. doi: 10.3109/08923978509047635.
It is wellknown that theophylline yields phenotypic changes on suppressor cells. In the present study we investigated the possibility that theophylline could directly induce a suppressor activity on a lymphocyte subpopulation. We observed that a short preincubation (120 min at 37 degrees C) with theophylline (1mM) activates human peripheral blood lymphocytes to suppress mitogenic response of autologous cells. This activity was not evident on a T cell subpopulation depleted of theophylline-sensitive (T-sens) lymphocytes. Theophylline mediated suppressor activity is only present in the Concanavalin A stimulated cultures, thus suggesting a synergism between Concanavalin A and theophylline in the expression of non specific suppression. Moreover we observed that after a 24 hrs preincubation of lymphocytes in complete culture medium there was a complete loss of theophylline-induced suppression. Such a preincubation time also produced a decrease in the theophylline-mediated enhancement of intracellular 3', 5' cyclic adenosine monophosphate levels and the impairment of E-rosette formation, suggesting that theophylline acts mainly on a "short-lived" suppressor lymphocyte subset.
众所周知，茶碱可使抑制细胞产生表型变化。在本研究中，我们探讨了茶碱能否直接诱导淋巴细胞亚群产生抑制活性的可能性。我们观察到，用茶碱（1mM）进行短暂预孵育（37℃下120分钟）可激活人外周血淋巴细胞，从而抑制自体细胞的有丝分裂反应。在去除对茶碱敏感（T-sens）淋巴细胞的T细胞亚群上，这种活性并不明显。茶碱介导的抑制活性仅存在于伴刀豆球蛋白A刺激的培养物中，这表明伴刀豆球蛋白A与茶碱在非特异性抑制表达中存在协同作用。此外，我们观察到，在完全培养基中对淋巴细胞进行24小时预孵育后，茶碱诱导的抑制作用完全丧失。这样的预孵育时间也导致茶碱介导的细胞内3'，5'环磷酸腺苷水平升高以及E-玫瑰花结形成受损，这表明茶碱主要作用于“短命”的抑制性淋巴细胞亚群。
