Department of Spine Surgery, Tianjin Union Medical Center, Tianjin, Hongqiao 300121, P.R. China.
Mol Med Rep. 2018 Apr;17(4):6016-6022. doi: 10.3892/mmr.2018.8561. Epub 2018 Feb 6.
The present study used a spinal cord injury (SCI) model to evaluate whether sparstolonin B was able to prevent SCI, and to investigate the underlying signaling mechanism. Sparstolonin B attenuated the SCI‑induced Batto, Beattie and Bresnahan score and water content in rats. Sparstolonin B attenuated the mRNA expression of proinflammatory cytokines interleukin (IL)‑18, IL‑6, IL‑1β, and IL‑23, decreased the levels of tumor necrosis factor‑α and interferon‑γ, and decreased caspase‑3 activity and apoptosis regulator Bax protein expression in SCI rats. Similarly, sparstolonin B inhibited monocyte chemoattractant protein‑1 mRNA levels, and Toll‑like receptor (TLR) 4, myeloid differentiation primary response protein MyD88 (MyD88) and nuclear factor (NF)‑κB protein levels in SCI rats. The present results suggested that sparstolonin B may attenuate SCI‑induced inflammation and apoptosis in rats by modulating the TLR4/MyD88/NF‑κB signaling pathway.
本研究采用脊髓损伤（SCI）模型来评估白首乌新苷 B 是否能够预防 SCI，并探讨其潜在的信号机制。白首乌新苷 B 可减轻 SCI 诱导的 Batto、Beattie 和 Bresnahan 评分以及大鼠脊髓组织含水量。白首乌新苷 B 可下调促炎细胞因子白细胞介素（IL）-18、IL-6、IL-1β 和 IL-23 的 mRNA 表达，降低肿瘤坏死因子-α和干扰素-γ的水平，并降低 SCI 大鼠中半胱氨酸天冬氨酸蛋白酶 3 活性和凋亡调节因子 Bax 蛋白表达。同样，白首乌新苷 B 可抑制 SCI 大鼠中单核细胞趋化蛋白-1 mRNA 水平，以及 Toll 样受体（TLR）4、髓样分化初级反应蛋白 MyD88（MyD88）和核因子（NF）-κB 蛋白水平。本研究结果表明，白首乌新苷 B 可能通过调节 TLR4/MyD88/NF-κB 信号通路来减轻 SCI 诱导的大鼠炎症和细胞凋亡。
