Department of Gastrointestinal and Hernia Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China.
Kunming Engineering Technology Center of Digestive Disease, Kunming, Yunnan 650032, P.R. China.
Mol Med Rep. 2018 Apr;17(4):5666-5675. doi: 10.3892/mmr.2018.8576. Epub 2018 Feb 8.
Lungs are the most common extra‑abdominal site of metastasis of colorectal cancer (CRC), in which long noncoding RNA (lncRNA) may serve a role. In the present study, a high‑throughput microarray assay was performed to detect lncRNA expression and identify novel targets for further study of lung metastasis in CRC. In the CRC tissues from patients with lung metastasis, 7,632 lncRNA (3,574 upregulated and 4,058 downregulated) and 6,185 mRNA (3,394 upregulated and 2,791 downregulated) were detected to be differentially expressed with a fold change ≥2 and P<0.05 compared with the CRC tissues without metastasis. A total of six differentially regulated lncRNA were confirmed by reverse transcription‑quantitative polymerase chain reaction in 20 pairs of CRC samples. Furthermore, gene ontology and pathway analysis were conducted to predict the possible roles of the identified mRNA. The upregulated mRNA were associated with cell division (biological processes), protein kinase B binding (molecular functions) and cellular components. The downregulated mRNA were associated with cell adhesion, platelet‑derived growth factor binding and membrane components. Pathway analysis determined that the upregulated mRNA were associated with the Wnt signaling pathway in the CRC tissues from patients with lung metastasis, while the downregulated mRNA were associated with the phosphoinositide 3‑kinase/Akt signaling pathway. The results of the present study suggested that differentially expressed lncRNA may be associated with lung metastasis and may provide insights into the biology and prevention of lung metastasis.
肺部是结直肠癌（CRC）最常见的腹腔外转移部位，长链非编码 RNA（lncRNA）可能在此过程中发挥作用。在本研究中，进行了高通量微阵列分析以检测 lncRNA 表达，并鉴定了用于进一步研究 CRC 肺转移的新靶标。在有肺转移的 CRC 组织中，与无转移的 CRC 组织相比，检测到 7,632 个 lncRNA（3,574 个上调和 4,058 个下调）和 6,185 个 mRNA（3,394 个上调和 2,791 个下调）表达差异显著，倍数变化≥2，且 P<0.05。在 20 对 CRC 样本中通过逆转录-定量聚合酶链反应验证了总共 6 个差异调节的 lncRNA。此外，进行了基因本体论和通路分析以预测鉴定的 mRNA 的可能作用。上调的 mRNA 与细胞分裂（生物学过程）、蛋白激酶 B 结合（分子功能）和细胞成分有关。下调的 mRNA 与细胞黏附、血小板衍生生长因子结合和膜成分有关。通路分析确定，在有肺转移的 CRC 组织中上调的 mRNA 与 Wnt 信号通路有关，而下调的 mRNA 与磷酸肌醇 3-激酶/Akt 信号通路有关。本研究的结果表明，差异表达的 lncRNA 可能与肺转移有关，并可能为肺转移的生物学和预防提供新的见解。
