Department of Pathology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, China.
Department of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
J Cell Biochem. 2018 Dec;119(12):9957-9966. doi: 10.1002/jcb.27319. Epub 2018 Aug 26.
Long noncoding RNA (lncRNA) plays an important regulatory role in tumorigenesis. This study aims to analyze the lncRNA-messenger RNA (mRNA) expression network and potential roles in colorectal cancer (CRC). The LncRNA expression profile was analyzed in CRC tissue by RNA sequencing and the functions of differentially expressed genes were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The lncRNA-mRNA network was predicted with bioinformatics. From the result, we identified 485 differential expression lncRNAs and 2383 mRNAs in CRC, GO, and KEGG analyses showed that the changes in lncRNAs were mainly associated with metabolism and transcription regulation that were different from mRNA function. Additionally, based on the predicted coexpression network, we identified that NONHSAT074176.2, downregulated in CRC tissue and cell lines, was a hub lncRNA in the development of CRC. Our results describe the lncRNA-mRNA network in detail and indicate that lncRNA NONHSAT074176.2 may be useful as a candidate diagnostic biomarker and may be a promising therapeutic target for CRC.
长链非编码 RNA（lncRNA）在肿瘤发生中发挥重要的调控作用。本研究旨在分析结直肠癌（CRC）中的 lncRNA-mRNA 表达网络及其潜在作用。通过 RNA 测序分析 CRC 组织中的 lncRNA 表达谱，并通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析差异表达基因的功能。通过生物信息学预测 lncRNA-mRNA 网络。结果表明，我们在 CRC 中鉴定出 485 个差异表达的 lncRNA 和 2383 个 mRNA，GO 和 KEGG 分析表明，lncRNA 的变化主要与代谢和转录调控有关，与 mRNA 功能不同。此外，基于预测的共表达网络，我们鉴定出在 CRC 组织和细胞系中下调的 NONHSAT074176.2 是 CRC 发展中的一个关键 lncRNA。我们的结果详细描述了 lncRNA-mRNA 网络，并表明 lncRNA NONHSAT074176.2 可能作为一种有前途的候选诊断生物标志物和 CRC 的治疗靶点。
