Department of Anesthesia, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
Department of Anesthesia, Zhengzhou Children's Hospital, Zhengzhou, Henan 450053, P.R. China.
Mol Med Rep. 2018 Apr;17(4):6033-6037. doi: 10.3892/mmr.2018.8597. Epub 2018 Feb 13.
Brain‑derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF) serves a significant role in neural protection by activating the phosphatidylinositol 3‑kinase (PI3K)/Akt signaling pathway, which also was associated with the neuroprotective the treatment with dexmedetomidine (DEX). The present study aimed to further explore whether treatment with DEX post‑IR increased the expression level of BDNF and VEGF in the rat brain. A total of 30 healthy, clean male Wistar rats were randomly divided into 3 experimental groups: Control group, ischemia/reperfusion (I/R) group and DEX treatment group. Subsequently, BDNF and VEGF mRNA and protein expression levels were analyzed. The results indicated that the mRNA expression levels of BDNF and VEGF were higher in the I/R and DEX groups compared with expression levels in the Control group at 6 h and 1 day post‑treatment; the levels of BNDF mRNA expression were higher in the DEX group compared with the I/R group. The levels of BDNF and VEGF protein expression in the I/R and DEX groups were also significantly higher compared with those in the Control group. I/R surgery significantly increased the expression of BDNF and VEGF protein DEX group at 6 h, day 1 and day 3 compared with expression levels in the I/R group. Results from the present study indicated that post‑surgical treatment with DEX may increase the expression of BDNF and VEGF following I/R, which may serve a role in nerve protection.
脑源性神经营养因子（BDNF）和血管内皮生长因子（VEGF）通过激活磷脂酰肌醇 3-激酶（PI3K）/Akt 信号通路在神经保护中发挥重要作用，这也与右美托咪定（DEX）的神经保护治疗有关。本研究旨在进一步探讨脑缺血再灌注（IR）后 DEX 治疗是否会增加大鼠脑中 BDNF 和 VEGF 的表达水平。将 30 只健康、清洁的雄性 Wistar 大鼠随机分为 3 个实验组：对照组、缺血/再灌注（I/R）组和 DEX 治疗组。随后，分析了 BDNF 和 VEGF 的 mRNA 和蛋白表达水平。结果表明，与对照组相比，I/R 组和 DEX 组在治疗后 6 h 和 1 天的 BDNF 和 VEGF mRNA 表达水平较高；DEX 组的 BNDF mRNA 表达水平高于 I/R 组。I/R 组和 DEX 组的 BDNF 和 VEGF 蛋白表达水平也明显高于对照组。与 I/R 组相比，DEX 组在 6 h、1 天和 3 天的 I/R 手术后 BDNF 和 VEGF 蛋白表达明显增加。本研究结果表明，术后 DEX 治疗可能会增加 I/R 后 BDNF 和 VEGF 的表达，从而在神经保护中发挥作用。
