Medical Examination Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, P.R. China.
Department of Teaching and Research of Diagnostics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, P.R. China.
Oncol Rep. 2018 Apr;39(4):1739-1746. doi: 10.3892/or.2018.6253. Epub 2018 Feb 7.
Human gastric cancer (GC) is the second most common cause of cancer-related deaths worldwide and is one of the most common metastatic cancers. Tumor proliferation, apoptosis, metastasis and invasion are important predictors of the invasiveness of GC and are key factors in cancer-induced death. Angiopoietin-like 4 (ANGPTL4) is a secreted protein that belongs to the angiopoietin (ANGPTL) family and is involved in the regulation of cancer metastasis. However, whether ANGPTL4 plays a role in the progression of GC remain unclear. In the present study, immunoreactivity of ANGPTL4 demonstrated that ANGPTL4 expression was upregulated in GC tissues with the development of GC. The siRNA targeting ANGPTL4 effectively knocked down ANGPTL4 in the SNU‑1 and BGC823 cell lines at the mRNA and protein levels. Following ANGPTL4 downregulation, the proliferation and invasion abilities of GC cell lines were suppressed as determined by MTT and Transwell assays, and cell apoptosis level and sensitivity to cisplatin were increased as determined by flow cytometry and MTT assay. In conclusion, these findings suggest that ANGPTL4 may be a new potential therapeutic target for GC.
人类胃癌（GC）是全球癌症相关死亡的第二大主要原因，也是最常见的转移性癌症之一。肿瘤增殖、凋亡、转移和侵袭是 GC 侵袭性的重要预测指标，也是癌症导致死亡的关键因素。血管生成素样 4（ANGPTL4）是一种分泌蛋白，属于血管生成素（ANGPTL）家族，参与调节癌症转移。然而，ANGPTL4 是否在 GC 的进展中发挥作用尚不清楚。在本研究中，免疫组织化学法检测到 ANGPTL4 的表达在 GC 组织中随着 GC 的发展而上调。siRNA 靶向 ANGPTL4 可有效下调 SNU-1 和 BGC823 细胞系中 ANGPTL4 的 mRNA 和蛋白水平。下调 ANGPTL4 后，通过 MTT 和 Transwell 测定法抑制 GC 细胞系的增殖和侵袭能力，通过流式细胞术和 MTT 测定法测定细胞凋亡水平和对顺铂的敏感性增加。综上所述，这些发现表明 ANGPTL4 可能是 GC 的一个新的潜在治疗靶点。
