• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

低强度超声联合低剂量卡铂在舌癌原位仓鼠模型中的作用：一项临床前研究。

Effects of low-intensity ultrasound combined with low-dose carboplatin in an orthotopic hamster model of tongue cancer: A preclinical study.

机构信息

Department of Forensic Medicine, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.

Department of Anatomy, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.

出版信息

Oncol Rep. 2018 Apr;39(4):1609-1618. doi: 10.3892/or.2018.6262. Epub 2018 Feb 13.

DOI:10.3892/or.2018.6262
PMID:29436690
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5868397/
Abstract

Low-intensity ultrasound (LIUS) combined with chemotherapy is an innovative modality for cancer treatment, but its effect on orthotopic carcinoma remains unknown. Our previous study revealed that LIUS enhanced the growth inhibitory effects of several chemotherapeutic drugs in nude mice with transplanted tumors. In the present study, we used 7,12-dimethylbenz(alpha)anthracene to induce orthotopic tongue carcinogenesis in hamsters. We used the first-line chemotherapy drug for tongue cancer, carboplatin (CBP) in combination with LIUS to investigate the synergistic effect. The results revealed that LIUS combined with low-dose CBP enhanced the inhibitory effects of CBP on tumor growth, prolonged survival, and did not increase the incidence of side-effects. It also enhanced the inherent DNA damage caused by CBP, suppressed the expression of the DNA repair proteins O6-methylguanine DNA methyltransferase (MGMT) and Chk1, and increased the expression of DNA damage-inducible protein GADD45α. Furthermore, compared with CBP alone, LIUS combined with CBP reduced the expression of cyclin D1 and cyclin B1, induced the expression of caspase-3, cleaved caspase-3, caspase-8, Bax, and Bak, and inhibited the expression of Bcl-2. Examination of clinical samples revealed that MGMT, Chk1, and Gadd45α were higher in OTSCC than in adjacent normal tissue. Hence, our results indicated that LIUS enhanced the ability of low-dose CBP to damage DNA in an orthotopic hamster model of tongue cancer, induced apoptosis, inhibited tumor growth and progression, while it did not increase the toxic side-effects of the drug, suggesting additional clinical benefits for patients treated with the combination of CBP with LIUS.

摘要

低强度超声（LIUS）联合化疗是一种治疗癌症的创新方法，但它对原位癌的影响尚不清楚。我们之前的研究表明，LIUS 增强了几种化疗药物在荷瘤裸鼠中的生长抑制作用。在本研究中，我们使用 7,12-二甲基苯并（α）蒽诱导仓鼠原位舌癌发生。我们使用舌癌一线化疗药物卡铂（CBP）联合 LIUS 来研究协同作用。结果表明，LIUS 联合低剂量 CBP 增强了 CBP 对肿瘤生长的抑制作用，延长了生存期，且未增加副作用的发生率。它还增强了 CBP 引起的固有 DNA 损伤，抑制了 DNA 修复蛋白 O6-甲基鸟嘌呤 DNA 甲基转移酶（MGMT）和 Chk1 的表达，并增加了 DNA 损伤诱导蛋白 GADD45α的表达。此外，与 CBP 单独使用相比，LIUS 联合 CBP 降低了 cyclin D1 和 cyclin B1 的表达，诱导了 caspase-3、cleaved caspase-3、caspase-8、Bax 和 Bak 的表达，并抑制了 Bcl-2 的表达。对临床样本的检查表明，MGMT、Chk1 和 Gadd45α 在 OTSCC 中的表达高于相邻正常组织。因此，我们的研究结果表明，LIUS 增强了低剂量 CBP 在原位仓鼠舌癌模型中损伤 DNA 的能力，诱导细胞凋亡，抑制肿瘤生长和进展，而不增加药物的毒性副作用，为接受 CBP 联合 LIUS 治疗的患者带来了额外的临床益处。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91e/5868397/6513feb4f79c/OR-39-04-1609-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91e/5868397/df05265a8c13/OR-39-04-1609-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91e/5868397/f7ce342b9c89/OR-39-04-1609-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91e/5868397/663728495d01/OR-39-04-1609-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91e/5868397/9e8f85008477/OR-39-04-1609-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91e/5868397/0e602c928985/OR-39-04-1609-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91e/5868397/6513feb4f79c/OR-39-04-1609-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91e/5868397/df05265a8c13/OR-39-04-1609-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91e/5868397/f7ce342b9c89/OR-39-04-1609-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91e/5868397/663728495d01/OR-39-04-1609-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91e/5868397/9e8f85008477/OR-39-04-1609-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91e/5868397/0e602c928985/OR-39-04-1609-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d91e/5868397/6513feb4f79c/OR-39-04-1609-g05.jpg

相似文献

1
Effects of low-intensity ultrasound combined with low-dose carboplatin in an orthotopic hamster model of tongue cancer: A preclinical study.低强度超声联合低剂量卡铂在舌癌原位仓鼠模型中的作用：一项临床前研究。
Oncol Rep. 2018 Apr;39(4):1609-1618. doi: 10.3892/or.2018.6262. Epub 2018 Feb 13.
2
Chemoprevention by Quercetin of Oral Squamous Cell Carcinoma by Suppression of the NF-κB Signaling Pathway in DMBA-treated Hamsters.槲皮素通过抑制二甲基苯并蒽处理的仓鼠中NF-κB信号通路对口腔鳞状细胞癌进行化学预防。
Anticancer Res. 2017 Aug;37(8):4041-4049. doi: 10.21873/anticanres.11789.
3
Carboplatin induces Fas (APO-1/CD95)-dependent apoptosis of human tongue carcinoma cells: sensitization for apoptosis by upregulation of FADD expression.卡铂诱导人舌癌细胞发生Fas（APO-1/CD95）依赖性凋亡：通过上调FADD表达使细胞对凋亡敏感。
Int J Cancer. 2003 Jul 10;105(5):593-600. doi: 10.1002/ijc.11133.
4
Epigenetic therapy using the histone deacetylase inhibitor for increasing therapeutic gain in oral cancer: prevention of radiation-induced oral mucositis and inhibition of chemical-induced oral carcinogenesis.使用组蛋白脱乙酰酶抑制剂的表观遗传疗法以提高口腔癌的治疗效果：预防放射性口腔黏膜炎并抑制化学诱导的口腔癌发生。
Carcinogenesis. 2009 Aug;30(8):1387-97. doi: 10.1093/carcin/bgp079. Epub 2009 Apr 7.
5
Chemopreventive effect of Mentha piperita on dimethylbenz[a]anthracene and formaldehyde-induced tongue carcinogenesis in mice (histological and immunohistochemical study).薄荷对二甲基苯并[a]蒽和甲醛诱导的小鼠舌癌发生的化学预防作用（组织学和免疫组织化学研究）
J Oral Pathol Med. 2014 Aug;43(7):484-91. doi: 10.1111/jop.12150. Epub 2014 Jan 23.
6
Emodin, aloe-emodin and rhein induced DNA damage and inhibited DNA repair gene expression in SCC-4 human tongue cancer cells.大黄素、芦荟大黄素和rhein 诱导 SCC-4 人舌癌细胞 DNA 损伤并抑制 DNA 修复基因的表达。
Anticancer Res. 2010 Mar;30(3):945-51.
7
Enhancement of anti-tumor effects of 5-fluorouracil on hepatocellular carcinoma by low-intensity ultrasound.低强度超声增强5-氟尿嘧啶对肝细胞癌的抗肿瘤作用
J Exp Clin Cancer Res. 2016 Apr 22;35:71. doi: 10.1186/s13046-016-0349-4.
8
Expression of apoptosis-related proteins and morphological changes in a rat tumor model of human small cell lung cancer prior to and after treatment with radiotherapy, carboplatin, or combined treatment.在人小细胞肺癌大鼠肿瘤模型中，放疗、卡铂或联合治疗前后凋亡相关蛋白的表达及形态学变化。
Virchows Arch. 2003 Apr;442(4):349-55. doi: 10.1007/s00428-003-0763-9. Epub 2003 Feb 21.
9
Effect of chemotherapy-induced DNA repair on oncolytic herpes simplex viral replication.化疗诱导的DNA修复对溶瘤单纯疱疹病毒复制的影响。
J Natl Cancer Inst. 2006 Jan 4;98(1):38-50. doi: 10.1093/jnci/djj003.
10
Combinatorial chemopreventive effect of butyric acid, nicotinamide and calcium glucarate against the 7,12-dimethylbenz(a)anthracene induced mouse skin tumorigenesis attained by enhancing the induction of intrinsic apoptotic events.丁酸钠、烟酰胺和葡萄糖醛酸钙通过增强内在凋亡事件的诱导来发挥联合化学预防作用，抑制 7,12-二甲基苯并（a）蒽诱导的小鼠皮肤肿瘤发生。
Chem Biol Interact. 2015 Jan 25;226:1-11. doi: 10.1016/j.cbi.2014.11.018. Epub 2014 Dec 3.

引用本文的文献

1
An ultrasound-activatable platinum prodrug for sono-sensitized chemotherapy.一种超声激活的顺铂前药用于声敏化疗。
Sci Adv. 2023 Jun 23;9(25):eadg5964. doi: 10.1126/sciadv.adg5964. Epub 2023 Jun 21.
2
Antitumorigenic Effect of Hsp90 Inhibitor SNX-2112 on Tongue Squamous Cell Carcinoma is Enhanced by Low-Intensity Ultrasound.Hsp90抑制剂SNX-2112对舌鳞状细胞癌的抗肿瘤作用因低强度超声而增强。
Onco Targets Ther. 2020 Aug 12;13:7907-7919. doi: 10.2147/OTT.S262174. eCollection 2020.
3
Ultrasound microbubble potentiated enhancement of hyperthermia-effect in tumours.

本文引用的文献

1
A Pleiotropic RNA-Binding Protein Controls Distinct Cell Cycle Checkpoints to Drive Resistance of p53-Defective Tumors to Chemotherapy.一种多效性RNA结合蛋白控制不同的细胞周期检查点，以驱动p53缺陷型肿瘤对化疗的抗性。
Cancer Cell. 2015 Dec 14;28(6):831. doi: 10.1016/j.ccell.2015.11.003.
2
Antiproliferative and Apoptosis-inducing Effect of exo-Protoporphyrin IX based Sonodynamic Therapy on Human Oral Squamous Cell Carcinoma.基于外原卟啉 IX 的声动力学疗法对人口腔鳞状细胞癌的抗增殖和促凋亡作用。
Sci Rep. 2017 Jan 19;7:40967. doi: 10.1038/srep40967.
3
Oral Squamous Cell Carcinoma: Current Treatment Strategies and Nanotechnology-Based Approaches for Prevention and Therapy.
超声微泡增强肿瘤热疗效果。
PLoS One. 2019 Dec 18;14(12):e0226475. doi: 10.1371/journal.pone.0226475. eCollection 2019.
口腔鳞状细胞癌：当前的治疗策略以及基于纳米技术的预防和治疗方法
Crit Rev Ther Drug Carrier Syst. 2016;33(4):363-400. doi: 10.1615/CritRevTherDrugCarrierSyst.2016016272.
4
Mitomycin C and decarbamoyl mitomycin C induce p53-independent p21WAF1/CIP1 activation.丝裂霉素C和去氨甲酰基丝裂霉素C诱导不依赖p53的p21WAF1/CIP1激活。
Int J Oncol. 2016 Nov;49(5):1815-1824. doi: 10.3892/ijo.2016.3703. Epub 2016 Sep 23.
5
A three-gene signature for prognosis in patients with MGMT promoter-methylated glioblastoma.一种用于预测MGMT启动子甲基化胶质母细胞瘤患者预后的三基因特征。
Oncotarget. 2016 Oct 25;7(43):69991-69999. doi: 10.18632/oncotarget.11726.
6
DNA REPAIR. Drugging DNA repair.DNA修复。对DNA修复进行药物治疗。
Science. 2016 Jun 3;352(6290):1178-9. doi: 10.1126/science.aab0958.
7
Enhancement of anti-tumor effects of 5-fluorouracil on hepatocellular carcinoma by low-intensity ultrasound.低强度超声增强5-氟尿嘧啶对肝细胞癌的抗肿瘤作用
J Exp Clin Cancer Res. 2016 Apr 22;35:71. doi: 10.1186/s13046-016-0349-4.
8
The release of Doxorubicin from liposomes monitored by MRI and triggered by a combination of US stimuli led to a complete tumor regression in a breast cancer mouse model.磁共振成像监测和超声联合刺激触发的脂质体阿霉素释放导致乳腺癌小鼠模型中的肿瘤完全消退。
J Control Release. 2016 May 28;230:57-63. doi: 10.1016/j.jconrel.2016.03.040. Epub 2016 Apr 2.
9
MMP-1/2 and TIMP-1/2 expression levels, and the levels of collagenous and elastic fibers correlate with disease progression in a hamster model of tongue cancer.基质金属蛋白酶-1/2和金属蛋白酶组织抑制因子-1/2的表达水平，以及胶原纤维和弹性纤维的水平与仓鼠舌癌模型中的疾病进展相关。
Oncol Lett. 2016 Jan;11(1):63-68. doi: 10.3892/ol.2015.3837. Epub 2015 Oct 29.
10
Cisplatin resistance and opportunities for precision medicine.顺铂耐药性与精准医学的机遇
Pharmacol Res. 2016 Apr;106:27-36. doi: 10.1016/j.phrs.2016.01.001. Epub 2016 Jan 22.