Huschka H G, Jalal M A, van der Helm D, Winkelmann G
J Bacteriol. 1986 Sep;167(3):1020-4. doi: 10.1128/jb.167.3.1020-1024.1986.
Recognition of ferric siderophores in Neurospora crassa was found to depend on the number and kind of N-acyl residues that surrounded the iron coordination center. In the coprogen series, uptake decreased in the order of coprogen, neocoprogen I, and neocoprogen II, indicating that gradual replacement of the N-transanhydromevalonyl groups by N-acetyl groups had an adverse effect on uptake. The reverse effect was observed in the ferrichrome series, where uptake decreased in the order of ferrichrysin, asperchrome D1, asperchrome B1, and ferrirubin. Configuration of the anhydromevalonyl group (cis or trans) in ferrichromes was also an important determinant in the recognition process. On the basis of uptake and inhibition studies, it is proposed that in ferrichromes part of the molecule (iron configuration and the N-acyl groups) is responsible for binding, whereas another (cyclic peptide ring) is involved in the subsequent process of transport.
研究发现，粗糙脉孢菌对三价铁载体的识别取决于围绕铁配位中心的N-酰基残基的数量和种类。在粪生螯合铁系列中，摄取量按粪生螯合铁、新粪生螯合铁I和新粪生螯合铁II的顺序降低，这表明N-反式脱水甲羟戊酰基逐渐被N-乙酰基取代对摄取有不利影响。在铁色素系列中观察到相反的效果，其中摄取量按铁黄菌素、曲霉色菌素D1、曲霉色菌素B1和铁红素的顺序降低。铁色素中脱水甲羟戊酰基的构型（顺式或反式）也是识别过程中的一个重要决定因素。基于摄取和抑制研究，有人提出，在铁色素中，分子的一部分（铁构型和N-酰基）负责结合，而另一部分（环肽环）参与随后的运输过程。
