College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871, China.
Peking-Tsinghua Center for Life Sciences, Beijing, 100871, China.
Angew Chem Int Ed Engl. 2018 Apr 3;57(15):3949-3953. doi: 10.1002/anie.201712614. Epub 2018 Mar 8.
Membrane voltage is an important biophysical signal that underlies intercellular electrical communications. A fluorescent voltage indicator is presented that enables the investigation of electrical signaling at high spatial resolution. The method is built upon the site-specific modification of microbial rhodopsin proteins with organic fluorophores, resulting in a hybrid indicator scaffold that is one of the most sensitive and fastest orange-colored voltage indicators developed to date. We applied this technique to optically map electrical connectivity in cultured cells, which revealed gap junction-mediated long-range coupling that spanned over hundreds of micrometers.
膜电压是一种重要的生物物理信号，它是细胞间电通信的基础。本文介绍了一种荧光电压指示剂，它可以实现高空间分辨率的电信号研究。该方法是基于在微生物视紫红质蛋白上进行的定点修饰，用有机荧光团对其进行修饰，从而得到一种杂交指示剂支架，它是迄今为止开发出的最灵敏、最快的橙色电压指示剂之一。我们将该技术应用于培养细胞的光学映射中，揭示了跨越数百微米的缝隙连接介导的长程耦合。
