Beck Connor, Zhang Diming, Gong Yiyang
Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
Curr Opin Biomed Eng. 2019 Dec;12:111-117. doi: 10.1016/j.cobme.2019.10.010. Epub 2019 Nov 9.
Genetically encoded voltage indicators report membrane voltage with high spatiotemporal resolution. Extensive recent efforts to improve the GEVIs' brightness, sensitivity, and kinetics have greatly increased the GEVIs' signal-to-noise performance over ten-fold and lowered their response time to the sub-millisecond regime. Such capabilities have broadened the GEVIs' ability to measure membrane voltage of neural populations at cellular resolution and , all at high speeds. The GEVIs' high voltage fidelity and fast response have revealed novel physiological phenomena in multiple neuroscientific applications. Such applications portend future targeted studies of voltage activity that take advantage of the GEVIs' ability to report rapid dynamics from genetically-targeted neural populations.
基因编码电压指示剂能够以高时空分辨率报告膜电压。近期为提高基因编码电压指示剂的亮度、灵敏度和动力学性能付出了大量努力，这使得其信噪比性能提高了十倍以上，并将响应时间缩短至亚毫秒级。这些能力拓宽了基因编码电压指示剂在细胞分辨率下高速测量神经群体膜电压的能力。基因编码电压指示剂的高电压保真度和快速响应在多个神经科学应用中揭示了新的生理现象。这些应用预示着未来将利用基因编码电压指示剂从基因靶向神经群体报告快速动态变化的能力，开展有针对性的电压活动研究。
