Automatic preparation of human serum samples for analysis of the drug enoximone and its sulphoxide metabolite using high-performance liquid chromatography.

A method for the estimation of the cardiotonic drug enoximone and its major sulphoxide metabolite, in serum, is described. The method uses a new technique for the preparation of biological material prior to the separation of analytes using high-performance liquid chromatography. This technique has been described as the automated sequential trace enrichment of dialysates (ASTED). The sample preparation process operates concurrently with the chromatographic separation. Aliquots of 500 microliters of serum are required for the analysis of serum from patients who have received a single 75-mg dose of enoximone. Within- and between-run coefficients of variation were found to be 2.8 and 3.9% for enoximone and 2.5 and 3.7% for enoximone sulphoxide. These values were obtained from estimating serum supplemented with enoximone and its metabolite, at concentrations of 0.5 and 1.0 mg/l, respectively.